Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines

Naseema M. Hoosein, Peter A. Kiener, Robert C. Curry, Lucio C. Rovati, Donnie K. McGilbra, Michael G. Brattain

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

The gastrointestinal hormone gastrin has been shown to stimulate the growth of normal colonic mucosa. To examine for a possible role of gastrin in the proliferation of cultured colon tumor cells, we have studied the effects of two gastrin receptor antagonists, proglumide and benzotript, and of antibodies to gastrin. We find that proglumide (50% effective concentration, 2 to 5 mm) and benzotript (50% effective concentration, 0.4 to 0.8 mm) inhibit the monolayer growth of six human colon cancer cell lines. Addition of exogenous gastrin abrogated the growth-inhibitory effect of proglumide. The anchorage-independent growth of colon carcinoma cells was also inhibited by the two gastrin antagonists. Also, a dose-dependent increase in carcinoembryonic antigen secretion was observed upon treatment with proglumide and benzotript in three cell lines examined. Half-maximal inhibition of labeled gastrin binding was observed at concentrations of 0.4 mM benzotript and 8.6 mm proglumide. In addition, antigastrin antiserum added to HCT 116 cells adapted to growth in serum-free medium resulted in a concentration-dependent inhibition of cellular proliferation. These data suggest that gastrin may function as an autocrine growth factor in colon carcinoma.

Original languageEnglish (US)
Pages (from-to)7179-7183
Number of pages5
JournalCancer Research
Volume48
StatePublished - Dec 1 1988

Fingerprint

Cholecystokinin B Receptor
Proglumide
Gastrins
Colon
Carcinoma
Cell Line
Antibodies
Growth
Cultured Tumor Cells
HCT116 Cells
Gastrointestinal Hormones
Carcinoembryonic Antigen
Serum-Free Culture Media
Colonic Neoplasms
Immune Sera
Intercellular Signaling Peptides and Proteins
Mucous Membrane
Cell Proliferation
benzotript

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Hoosein, N. M., Kiener, P. A., Curry, R. C., Rovati, L. C., McGilbra, D. K., & Brattain, M. G. (1988). Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines. Cancer Research, 48, 7179-7183.

Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines. / Hoosein, Naseema M.; Kiener, Peter A.; Curry, Robert C.; Rovati, Lucio C.; McGilbra, Donnie K.; Brattain, Michael G.

In: Cancer Research, Vol. 48, 01.12.1988, p. 7179-7183.

Research output: Contribution to journalArticle

Hoosein, NM, Kiener, PA, Curry, RC, Rovati, LC, McGilbra, DK & Brattain, MG 1988, 'Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines', Cancer Research, vol. 48, pp. 7179-7183.
Hoosein NM, Kiener PA, Curry RC, Rovati LC, McGilbra DK, Brattain MG. Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines. Cancer Research. 1988 Dec 1;48:7179-7183.
Hoosein, Naseema M. ; Kiener, Peter A. ; Curry, Robert C. ; Rovati, Lucio C. ; McGilbra, Donnie K. ; Brattain, Michael G. / Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines. In: Cancer Research. 1988 ; Vol. 48. pp. 7179-7183.
@article{4e367945be1b48b794fc69bc696e1128,
title = "Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines",
abstract = "The gastrointestinal hormone gastrin has been shown to stimulate the growth of normal colonic mucosa. To examine for a possible role of gastrin in the proliferation of cultured colon tumor cells, we have studied the effects of two gastrin receptor antagonists, proglumide and benzotript, and of antibodies to gastrin. We find that proglumide (50{\%} effective concentration, 2 to 5 mm) and benzotript (50{\%} effective concentration, 0.4 to 0.8 mm) inhibit the monolayer growth of six human colon cancer cell lines. Addition of exogenous gastrin abrogated the growth-inhibitory effect of proglumide. The anchorage-independent growth of colon carcinoma cells was also inhibited by the two gastrin antagonists. Also, a dose-dependent increase in carcinoembryonic antigen secretion was observed upon treatment with proglumide and benzotript in three cell lines examined. Half-maximal inhibition of labeled gastrin binding was observed at concentrations of 0.4 mM benzotript and 8.6 mm proglumide. In addition, antigastrin antiserum added to HCT 116 cells adapted to growth in serum-free medium resulted in a concentration-dependent inhibition of cellular proliferation. These data suggest that gastrin may function as an autocrine growth factor in colon carcinoma.",
author = "Hoosein, {Naseema M.} and Kiener, {Peter A.} and Curry, {Robert C.} and Rovati, {Lucio C.} and McGilbra, {Donnie K.} and Brattain, {Michael G.}",
year = "1988",
month = "12",
day = "1",
language = "English (US)",
volume = "48",
pages = "7179--7183",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",

}

TY - JOUR

T1 - Antiproliferative effects of gastrin receptor antagonists and antibodies to gastrin on human colon carcinoma cell lines

AU - Hoosein, Naseema M.

AU - Kiener, Peter A.

AU - Curry, Robert C.

AU - Rovati, Lucio C.

AU - McGilbra, Donnie K.

AU - Brattain, Michael G.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - The gastrointestinal hormone gastrin has been shown to stimulate the growth of normal colonic mucosa. To examine for a possible role of gastrin in the proliferation of cultured colon tumor cells, we have studied the effects of two gastrin receptor antagonists, proglumide and benzotript, and of antibodies to gastrin. We find that proglumide (50% effective concentration, 2 to 5 mm) and benzotript (50% effective concentration, 0.4 to 0.8 mm) inhibit the monolayer growth of six human colon cancer cell lines. Addition of exogenous gastrin abrogated the growth-inhibitory effect of proglumide. The anchorage-independent growth of colon carcinoma cells was also inhibited by the two gastrin antagonists. Also, a dose-dependent increase in carcinoembryonic antigen secretion was observed upon treatment with proglumide and benzotript in three cell lines examined. Half-maximal inhibition of labeled gastrin binding was observed at concentrations of 0.4 mM benzotript and 8.6 mm proglumide. In addition, antigastrin antiserum added to HCT 116 cells adapted to growth in serum-free medium resulted in a concentration-dependent inhibition of cellular proliferation. These data suggest that gastrin may function as an autocrine growth factor in colon carcinoma.

AB - The gastrointestinal hormone gastrin has been shown to stimulate the growth of normal colonic mucosa. To examine for a possible role of gastrin in the proliferation of cultured colon tumor cells, we have studied the effects of two gastrin receptor antagonists, proglumide and benzotript, and of antibodies to gastrin. We find that proglumide (50% effective concentration, 2 to 5 mm) and benzotript (50% effective concentration, 0.4 to 0.8 mm) inhibit the monolayer growth of six human colon cancer cell lines. Addition of exogenous gastrin abrogated the growth-inhibitory effect of proglumide. The anchorage-independent growth of colon carcinoma cells was also inhibited by the two gastrin antagonists. Also, a dose-dependent increase in carcinoembryonic antigen secretion was observed upon treatment with proglumide and benzotript in three cell lines examined. Half-maximal inhibition of labeled gastrin binding was observed at concentrations of 0.4 mM benzotript and 8.6 mm proglumide. In addition, antigastrin antiserum added to HCT 116 cells adapted to growth in serum-free medium resulted in a concentration-dependent inhibition of cellular proliferation. These data suggest that gastrin may function as an autocrine growth factor in colon carcinoma.

UR - http://www.scopus.com/inward/record.url?scp=0024269135&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024269135&partnerID=8YFLogxK

M3 - Article

C2 - 3191491

AN - SCOPUS:0024269135

VL - 48

SP - 7179

EP - 7183

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

ER -