Antiproliferative activity of shark cartilage with and without tumor necrosis factor-α in human umbilical vein endothelium

Timothy R McGuire, Peter W. Kazakoff, Eric B. Hoie, Margery A. Fienhold

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We evaluated the antiangiogenic activity of shark cartilage, tumor necrosis factor-α (TNF-α), and a combination of the two using a human umbilical vein endothelial cell proliferation assay Proliferation of endothelium is a hallmark of angiogenesis, and inhibition of endothelial cell proliferation indicates potential antiangiogenic activity. Shark cartilage produced a concentration-dependent decline in endothelial cell 3H-thymidine incorporation. This activity was heat stable and was found in molecular weight fractions of less than 10 kd. The antiproliferative effect of shark cartilage was specific for vascular endothelium and did not affect the proliferative rate of human astrocytoma cells or human skin fibroblasts. Shark cartilage at a concentration of 500 μg/ml and TNF-α at a concentration of 10 ng/ml reduced endothelial cell proliferation by 32% and 29%, respectively. Treatment of endothelial cells with the combination of shark cartilage and TNF-α resulted in a 44% reduction in endothelial cell proliferation. The isolation and identification of the active components of shark cartilage is continuing.

Original languageEnglish (US)
Pages (from-to)237-244
Number of pages8
JournalPharmacotherapy
Volume16
Issue number2 I
StatePublished - Mar 27 1996

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Sharks
Umbilical Veins
Cartilage
Endothelium
Endothelial Cells
Cell Proliferation
Tumor Necrosis Factor-alpha
Human Umbilical Vein Endothelial Cells
Astrocytoma
Vascular Endothelium
Thymidine
human TNF protein
Fibroblasts
Hot Temperature
Molecular Weight
Skin

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Antiproliferative activity of shark cartilage with and without tumor necrosis factor-α in human umbilical vein endothelium. / McGuire, Timothy R; Kazakoff, Peter W.; Hoie, Eric B.; Fienhold, Margery A.

In: Pharmacotherapy, Vol. 16, No. 2 I, 27.03.1996, p. 237-244.

Research output: Contribution to journalArticle

McGuire, Timothy R ; Kazakoff, Peter W. ; Hoie, Eric B. ; Fienhold, Margery A. / Antiproliferative activity of shark cartilage with and without tumor necrosis factor-α in human umbilical vein endothelium. In: Pharmacotherapy. 1996 ; Vol. 16, No. 2 I. pp. 237-244.
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