Antiproliferation properties of grain sorghum dry distiller's grain lipids in caco-2 cells

Richard Zbasnik, Timothy P Carr, Curtis Weller, Keum Taek Hwang, Lijun Wang, Susan Cuppett, Vicki L Schlegel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Antiproliferative properties of lipids extracted from grain sorghum (GS) dry distiller's grain (DDG) were analyzed to determine the feasibility of developing GS coproducts as a source for human health dietary ingredients. The lipid extract of GS-DDG was delivered to human colon carcinoma (Caco-2) cells by solubilizing 0-1000 μg/mL of GS-DDG lipids in 100 μg/mL increments with micelles. A significant reduction in cell viability (25-50%) resulted at treatment levels of 400-1000 μg/mL GS-DDG lipids (p < 0.05). Alternatively, total protein levels of cells treated with 400, 500, and 600,μ/mL of GS-DDG lipid were not significantly different from the control, indicating cell growth during the treatment period. Total cell counts for the control were not significantly different from the GS-DDG lipid treated cells, but dead cell counts increased by ~10% for the latter sample with a concomitant increase of the Intercellular protein lactate dehydrogenase leakage (30-40%) in the medium. Preliminary analysis by the fluorescence-activated cell method (FACs) demonstrated that nonviable cells were in either the early apoptotic, late apoptotic, or necrotic stage post-treatment with 400, 500, and 600μg/mL GS-DDG lipids. Physiochemical characterization of the GS-DDG lipids used for the antiproliferation study showed the presence of vitamin E (predominantly y-tocopherol), triacylglycerides (predominantly linoleic acid), policosanols, aldehydes, and sterols (predominantly campesterol and stigmasterol), each of which or as synergistic/additive group of constituents may be responsible for the antiproliferative effect.

Original languageEnglish (US)
Pages (from-to)10435-10441
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number21
DOIs
StatePublished - Nov 11 2009

Fingerprint

Sorghum
Caco-2 Cells
distillers grains
grain sorghum
Lipids
lipids
cells
policosanol
Cell Count
Stigmasterol
campesterol
coproducts
Tocopherols
stigmasterol
Micelles
micelles
Sterols
Linoleic Acid
lactate dehydrogenase
Vitamin E

Keywords

  • Antiproliferation
  • Caco-2 cells
  • Dry distiller's grain
  • Ethanol
  • Grain sorghum

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Antiproliferation properties of grain sorghum dry distiller's grain lipids in caco-2 cells. / Zbasnik, Richard; Carr, Timothy P; Weller, Curtis; Hwang, Keum Taek; Wang, Lijun; Cuppett, Susan; Schlegel, Vicki L.

In: Journal of Agricultural and Food Chemistry, Vol. 57, No. 21, 11.11.2009, p. 10435-10441.

Research output: Contribution to journalArticle

Zbasnik, Richard ; Carr, Timothy P ; Weller, Curtis ; Hwang, Keum Taek ; Wang, Lijun ; Cuppett, Susan ; Schlegel, Vicki L. / Antiproliferation properties of grain sorghum dry distiller's grain lipids in caco-2 cells. In: Journal of Agricultural and Food Chemistry. 2009 ; Vol. 57, No. 21. pp. 10435-10441.
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