Antimicrobial Properties of Graphene Oxide Nanosheets

Why Size Matters

François Perreault, Andreia Fonseca De Faria, Siamak Nejati, Menachem Elimelech

Research output: Contribution to journalArticle

308 Citations (Scopus)

Abstract

Graphene oxide (GO) is a promising material for the development of antimicrobial surfaces due to its contact-based antimicrobial activity. However, the relationship between GO physicochemical properties and its antimicrobial activity has yet to be elucidated. In this study, we investigated the size-dependency of GO antimicrobial activity using the Gram-negative bacteria Escherichia coli. GO suspensions of average sheet area ranging from 0.01 to 0.65 μm2 were produced and their antimicrobial activity evaluated in cell suspensions or as a model GO surface coating. The antimicrobial activity of GO surface coatings increased 4-fold when GO sheet area decreased from 0.65 to 0.01 μm2. The higher antimicrobial effect of smaller GO sheets is attributed to oxidative mechanisms associated with the higher defect density of smaller sheets. In contrast, in suspension assays, GO interacted with bacteria in a cell entrapment mechanism; in this case, the antimicrobial effect of GO increased with increasing sheet area, with apparent complete inactivation observed for the 0.65 μm2 sheets after a 3 h exposure. However, cell inactivation by GO entrapment was reversible and all initially viable cells could be recovered when separated from GO sheets by sonication. These findings provide useful guidelines for future development of graphene-based antimicrobial surface coatings, where smaller sheet sizes can increase the antimicrobial activity of the material. Our study further emphasizes the importance of an accurate assessment of the antimicrobial effect of nanomaterials when used for antimicrobial surface design.

Original languageEnglish (US)
Pages (from-to)7226-7236
Number of pages11
JournalACS Nano
Volume9
Issue number7
DOIs
StatePublished - Jul 28 2015
Externally publishedYes

Fingerprint

Graphite
Nanosheets
Oxides
Graphene
graphene
oxides
Suspensions
entrapment
cells
coatings
Coatings
deactivation
bacteria
Bacteria
Sonication
Defect density
Escherichia
Nanostructured materials
Escherichia coli
Assays

Keywords

  • antimicrobial surfaces
  • bacterial toxicity
  • glutathione
  • graphene oxide
  • oxidative damage

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Antimicrobial Properties of Graphene Oxide Nanosheets : Why Size Matters. / Perreault, François; De Faria, Andreia Fonseca; Nejati, Siamak; Elimelech, Menachem.

In: ACS Nano, Vol. 9, No. 7, 28.07.2015, p. 7226-7236.

Research output: Contribution to journalArticle

Perreault, François ; De Faria, Andreia Fonseca ; Nejati, Siamak ; Elimelech, Menachem. / Antimicrobial Properties of Graphene Oxide Nanosheets : Why Size Matters. In: ACS Nano. 2015 ; Vol. 9, No. 7. pp. 7226-7236.
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