Antigens Expressed on NIH 3T3 Cells Following Transformation with DNA from a Human Pancreatic Tumor

Michael A. Hollingsworth, Lorita M. Rebellato, James W. Moore, Olivera J. Finn, Richard S. Metzgar

Research output: Contribution to journalArticle

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Abstract

This report documents that the pancreatic adenocarcinoma cell line, HPAF, contains oncogene activity detected by transformation of NIH 3T3 cells through transfection with HPAF DNA. The HPAF transfected NIH 3T3 cells do not contain oncogenes homologous with c-H-ras c-K-ras,c-N-ras, v-fms, c-myb, c-sis, v-fgr, c-mos, c-myc, c-fos, v-fes v-src, v-erb A, v-erb B, c-N-myc, v-raf, or v-abl other than the endogenous mouse genes. The transfectants do express proteins detected by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis which were not found in nontransfected NIH 3T3 cells. Monoclonal antibodies raised against the transfectants recognize proteins not found in untransfected NIH 3T3 cells that are antigenically identical to proteins found in the HPAF cells. These antigens are also detected on six other human pancreatic adenocarcinoma cell lines but show a much more restricted distribution on lymphoblastoid, melanoma, prostatic carcinoma, and normal skin fibroblasT cell lines. copyright.

Original languageEnglish (US)
Pages (from-to)2482-2487
Number of pages6
JournalCancer Research
Volume46
Issue number5
StatePublished - May 1 1986

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NIH 3T3 Cells
Antigens
DNA
Oncogenes
Cell Line
Neoplasms
Adenocarcinoma
Proteins
Sodium Dodecyl Sulfate
Transfection
Polyacrylamide Gel Electrophoresis
Melanoma
Fibroblasts
Monoclonal Antibodies
Carcinoma
Skin
Genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Hollingsworth, M. A., Rebellato, L. M., Moore, J. W., Finn, O. J., & Metzgar, R. S. (1986). Antigens Expressed on NIH 3T3 Cells Following Transformation with DNA from a Human Pancreatic Tumor. Cancer Research, 46(5), 2482-2487.

Antigens Expressed on NIH 3T3 Cells Following Transformation with DNA from a Human Pancreatic Tumor. / Hollingsworth, Michael A.; Rebellato, Lorita M.; Moore, James W.; Finn, Olivera J.; Metzgar, Richard S.

In: Cancer Research, Vol. 46, No. 5, 01.05.1986, p. 2482-2487.

Research output: Contribution to journalArticle

Hollingsworth, MA, Rebellato, LM, Moore, JW, Finn, OJ & Metzgar, RS 1986, 'Antigens Expressed on NIH 3T3 Cells Following Transformation with DNA from a Human Pancreatic Tumor', Cancer Research, vol. 46, no. 5, pp. 2482-2487.
Hollingsworth, Michael A. ; Rebellato, Lorita M. ; Moore, James W. ; Finn, Olivera J. ; Metzgar, Richard S. / Antigens Expressed on NIH 3T3 Cells Following Transformation with DNA from a Human Pancreatic Tumor. In: Cancer Research. 1986 ; Vol. 46, No. 5. pp. 2482-2487.
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