Antigenic characterization of foot-and-mouth disease virus serotype Asia1 field isolates using polyclonal and monoclonal antibodies

A. Sanyal, Channabasavaiah B Gurumurthy, R. Venkataramanan, D. Hemadri, C. Tosh

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Foot-and-mouth disease virus (FMDV) serotype Asia1 field isolates (n=100) were compared using a panel of 11 monoclonal antibodies (Mab) in sandwich ELISA. The majority (over 89%) of the isolates showed either homologous (76% and above reactivity) or reduced affinity (20-75% reactivity) for the Mabs 2A, 13, 40, 34 and 81, suggesting that these Mab binding epitopes are conserved, whereas a more variable reactivity was observed for the Mabs B3, 1A, 24, 72, 82 and 89. Polyclonal relationship ('r' value) of the field isolates in liquid phase blocking (LPB) ELISA was examined, and the mean 'r' value was 0.62 relative to vaccine virus IND 63/72. Some of the field isolates (n=34) were tested in virus neutralization test (VNT) and showed an 'r' value of >0.40. Although a minor antigenic difference was observed in the Mab profiling study, there has not been large antigenic divergence between reference virus and field viruses, thereby providing evidence of wide antigenic coverage of the vaccine strain.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalVeterinary Microbiology
Volume93
Issue number1
DOIs
StatePublished - May 2 2003

Fingerprint

Foot-and-Mouth Disease Virus
Foot-and-mouth disease virus
polyclonal antibodies
monoclonal antibodies
serotypes
Monoclonal Antibodies
Viruses
viruses
enzyme-linked immunosorbent assay
vaccines
Vaccines
Enzyme-Linked Immunosorbent Assay
neutralization tests
sandwiches
Neutralization Tests
epitopes
Epitopes
liquids
Serogroup

Keywords

  • Antigenic analysis
  • Field isolates
  • Foot-and-mouth disease virus
  • Monoclonal antibodies
  • Serotype Asia1

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Antigenic characterization of foot-and-mouth disease virus serotype Asia1 field isolates using polyclonal and monoclonal antibodies. / Sanyal, A.; Gurumurthy, Channabasavaiah B; Venkataramanan, R.; Hemadri, D.; Tosh, C.

In: Veterinary Microbiology, Vol. 93, No. 1, 02.05.2003, p. 1-11.

Research output: Contribution to journalArticle

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