Antigen-responsive CD4+ T cells from C3H mice chronically infected with Leishmania amazonensis are impaired in the transition to an effector phenotype

Amanda E. Ramer, Yannick F. Vanloubbeeck, Douglas E. Jones

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

C3HeB/FeJ mice challenged with Leishmania major develop a polarized th1 response and subsequently heal, whereas Leishmania amazonensis challenge leads to clironic lesions with high parasite loads at 10 weeks postinfection. In this study, a comparison of draining lymph node cells from L. amazonensis- and L. major-infected mice at 10 weeks postinfection showed equivalent percentages of effector/memory phenotype CD44hi CD4+ T cells producing interleukin-2 (IL-2) and proliferating after antigen stimulation. However, these cells isolated from L. amazonensis-infected mice were not skewed toward either a Th1 or Th2 phenotype in vivo, as evidenced by their unbiased Th1/Th2 transcription factor mRNA profile. In vivo antigen stimulation with added IL-12 failed to enhance gamma interferon (IFN-γ) production of CD4+ T cells from L. amazonensis-infected mice. Antigen stimulation of CD4+ T cells from L. amazonensis-infected mice in vitro, in the presence of IL-12 resulted in production of only 10 to 15% of the IFN-γ produced by T cells from L. major-infected mice under identical conditions. These results suggest that the CD4+ T-cell response during chronic L. amazonensis infection is limited during the transition from an early activated CD4+ T-cell population to an effector cell population and demonstrate that these T cells have an intrinsic defect beyond the presence or absence of IL-12 during antigen stimulation.

Original languageEnglish (US)
Pages (from-to)1547-1554
Number of pages8
JournalInfection and immunity
Volume74
Issue number3
DOIs
StatePublished - Mar 1 2006

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CD4 Antigens
Inbred C3H Mouse
Leishmania
T-Lymphocytes
Phenotype
Leishmania major
Interleukin-12
Antigens
Parasite Load
Interferons
Population
Interferon-gamma
Interleukin-2
Transcription Factors
Lymph Nodes
Messenger RNA
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Antigen-responsive CD4+ T cells from C3H mice chronically infected with Leishmania amazonensis are impaired in the transition to an effector phenotype. / Ramer, Amanda E.; Vanloubbeeck, Yannick F.; Jones, Douglas E.

In: Infection and immunity, Vol. 74, No. 3, 01.03.2006, p. 1547-1554.

Research output: Contribution to journalArticle

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