Antifungal activity of endosequence root repair material and mineral trioxide aggregate

Fahd Alsalleeh, Nicole Chung, Lane Stephenson

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction The purpose of this study was to investigate the antifungal activity of Endosequence Root Repair Material (ERRM; Brasseler USA, Savannah, GA) as compared with mineral trioxide aggregate (MTA) using Candida albicans.

Methods All materials were packed into sterilized intravenous tubing to obtain standardized samples and allowed to set for 3 or 24 hours and then exposed to a suspension of C. albicans for incubations of 24 or 48 hours. To analyze the mechanisms of the material's antifungal activity, additional samples of each test material were prepared in the same manner and allowed to set for 24 hours; these were then incubated in a culture medium for 24 hours. The pH of each conditioned media was measured before transferring to wells containing C. albicans. The development of biofilm was analyzed after 24 and 48 hours with 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-([phenyl amino] carbonyl)-2H-tetrazolium hydroxide reduction assay.

Results Materials in both experimental groups significantly limited biofilm formation at each interval (ie, 24 and 48 hours). After incubating for a 24-hour period in the presence of C. albicans, ERRM in both experimental groups showed a reduction in biofilm formation that was statistically significant in comparison with MTA. However, when set for 24 hours and incubated for 48 hours, gray MTA and white MTA showed a more substantial reduction in biofilm formation than comparable samples of ERRM. Cultured media conditioned with test materials showed statistically significant antifungal biofilm activity after 48 hours.

Conclusions All materials tested have comparable antifungal biofilm activity. It appeared that changing the environment, such as the pH, contributed to this activity.

Original languageEnglish (US)
Pages (from-to)1815-1819
Number of pages5
JournalJournal of Endodontics
Volume40
Issue number11
DOIs
StatePublished - Nov 1 2014

Fingerprint

Biofilms
Candida albicans
Conditioned Culture Medium
Culture Media
mineral trioxide aggregate
EndoSequence root repair material
Suspensions

Keywords

  • C. albicans
  • Endosequence Root Repair Material
  • fungal biofilm
  • mineral trioxide aggregate

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antifungal activity of endosequence root repair material and mineral trioxide aggregate. / Alsalleeh, Fahd; Chung, Nicole; Stephenson, Lane.

In: Journal of Endodontics, Vol. 40, No. 11, 01.11.2014, p. 1815-1819.

Research output: Contribution to journalArticle

Alsalleeh, Fahd ; Chung, Nicole ; Stephenson, Lane. / Antifungal activity of endosequence root repair material and mineral trioxide aggregate. In: Journal of Endodontics. 2014 ; Vol. 40, No. 11. pp. 1815-1819.
@article{075f07febc24479b9df06680ef61940f,
title = "Antifungal activity of endosequence root repair material and mineral trioxide aggregate",
abstract = "Introduction The purpose of this study was to investigate the antifungal activity of Endosequence Root Repair Material (ERRM; Brasseler USA, Savannah, GA) as compared with mineral trioxide aggregate (MTA) using Candida albicans.Methods All materials were packed into sterilized intravenous tubing to obtain standardized samples and allowed to set for 3 or 24 hours and then exposed to a suspension of C. albicans for incubations of 24 or 48 hours. To analyze the mechanisms of the material's antifungal activity, additional samples of each test material were prepared in the same manner and allowed to set for 24 hours; these were then incubated in a culture medium for 24 hours. The pH of each conditioned media was measured before transferring to wells containing C. albicans. The development of biofilm was analyzed after 24 and 48 hours with 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-([phenyl amino] carbonyl)-2H-tetrazolium hydroxide reduction assay.Results Materials in both experimental groups significantly limited biofilm formation at each interval (ie, 24 and 48 hours). After incubating for a 24-hour period in the presence of C. albicans, ERRM in both experimental groups showed a reduction in biofilm formation that was statistically significant in comparison with MTA. However, when set for 24 hours and incubated for 48 hours, gray MTA and white MTA showed a more substantial reduction in biofilm formation than comparable samples of ERRM. Cultured media conditioned with test materials showed statistically significant antifungal biofilm activity after 48 hours.Conclusions All materials tested have comparable antifungal biofilm activity. It appeared that changing the environment, such as the pH, contributed to this activity.",
keywords = "C. albicans, Endosequence Root Repair Material, fungal biofilm, mineral trioxide aggregate",
author = "Fahd Alsalleeh and Nicole Chung and Lane Stephenson",
year = "2014",
month = "11",
day = "1",
doi = "10.1016/j.joen.2014.08.002",
language = "English (US)",
volume = "40",
pages = "1815--1819",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "11",

}

TY - JOUR

T1 - Antifungal activity of endosequence root repair material and mineral trioxide aggregate

AU - Alsalleeh, Fahd

AU - Chung, Nicole

AU - Stephenson, Lane

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Introduction The purpose of this study was to investigate the antifungal activity of Endosequence Root Repair Material (ERRM; Brasseler USA, Savannah, GA) as compared with mineral trioxide aggregate (MTA) using Candida albicans.Methods All materials were packed into sterilized intravenous tubing to obtain standardized samples and allowed to set for 3 or 24 hours and then exposed to a suspension of C. albicans for incubations of 24 or 48 hours. To analyze the mechanisms of the material's antifungal activity, additional samples of each test material were prepared in the same manner and allowed to set for 24 hours; these were then incubated in a culture medium for 24 hours. The pH of each conditioned media was measured before transferring to wells containing C. albicans. The development of biofilm was analyzed after 24 and 48 hours with 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-([phenyl amino] carbonyl)-2H-tetrazolium hydroxide reduction assay.Results Materials in both experimental groups significantly limited biofilm formation at each interval (ie, 24 and 48 hours). After incubating for a 24-hour period in the presence of C. albicans, ERRM in both experimental groups showed a reduction in biofilm formation that was statistically significant in comparison with MTA. However, when set for 24 hours and incubated for 48 hours, gray MTA and white MTA showed a more substantial reduction in biofilm formation than comparable samples of ERRM. Cultured media conditioned with test materials showed statistically significant antifungal biofilm activity after 48 hours.Conclusions All materials tested have comparable antifungal biofilm activity. It appeared that changing the environment, such as the pH, contributed to this activity.

AB - Introduction The purpose of this study was to investigate the antifungal activity of Endosequence Root Repair Material (ERRM; Brasseler USA, Savannah, GA) as compared with mineral trioxide aggregate (MTA) using Candida albicans.Methods All materials were packed into sterilized intravenous tubing to obtain standardized samples and allowed to set for 3 or 24 hours and then exposed to a suspension of C. albicans for incubations of 24 or 48 hours. To analyze the mechanisms of the material's antifungal activity, additional samples of each test material were prepared in the same manner and allowed to set for 24 hours; these were then incubated in a culture medium for 24 hours. The pH of each conditioned media was measured before transferring to wells containing C. albicans. The development of biofilm was analyzed after 24 and 48 hours with 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-([phenyl amino] carbonyl)-2H-tetrazolium hydroxide reduction assay.Results Materials in both experimental groups significantly limited biofilm formation at each interval (ie, 24 and 48 hours). After incubating for a 24-hour period in the presence of C. albicans, ERRM in both experimental groups showed a reduction in biofilm formation that was statistically significant in comparison with MTA. However, when set for 24 hours and incubated for 48 hours, gray MTA and white MTA showed a more substantial reduction in biofilm formation than comparable samples of ERRM. Cultured media conditioned with test materials showed statistically significant antifungal biofilm activity after 48 hours.Conclusions All materials tested have comparable antifungal biofilm activity. It appeared that changing the environment, such as the pH, contributed to this activity.

KW - C. albicans

KW - Endosequence Root Repair Material

KW - fungal biofilm

KW - mineral trioxide aggregate

UR - http://www.scopus.com/inward/record.url?scp=84908283889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84908283889&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2014.08.002

DO - 10.1016/j.joen.2014.08.002

M3 - Article

VL - 40

SP - 1815

EP - 1819

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 11

ER -