Antibody responses of Porphyromonas gingivalis infected gingivitis and periodontitis subjects

E. Gemmell, B. Polak, Richard A Reinhardt, J. Eccleston, G. J. Seymour

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Porphyrornonas gingivalis demonstrates a strong association with adult periodontitis although some individuals with the infection do not experience attachment loss. Therefore differences in the immune response to this organism may be of importance to the outcome of the disease. OBJECTIVE: The aim of this study was to determine whether P. gingivalis positive subjects with and without periodontal breakdown, reacted differently to P. gingivolis antigens as assessed by the pattern of serum antibody reactivity. MATERIALS AND METHODS: Two highly defined groups Of subjects were chosen for this study. Both demOnstrated p gingivalis in their plaque and both had responded to P. gingivolis as shown by the presence of serum antibodies* The two groups differed only in their apparent clinical susceptibility to periodontal breakd˜wn. Western blots of P. gingivolis membrane antigens were probed with sera from the two groups to determine their reactivity to specific antigens. RESULTS: Analysis Of the immunoblots showed that there were no differences in either the total numbers of bands, Or bands recognized by the majority Of subjects in the gingivitis and adult periodontitis groups. There were however, four bands recognized by the majority of the gingivitis group and not by the majority Of the adult periodontitis group, there being a significant difference (P = 0.03) in the recognition of the 91.4‐kDa antigen band* A further five antigens Of lower molecular weight were seen by the majority of the adult periodontitis group and not by the majority of the gingivitis group. When sera were tested against purified p*gingivalis Lps, the results indicated that the five antigens seen by the majority Of the adult periodontitis group had molecular weights which were in the range exhibited by the Lps antigens. CONCLUSION: These results suggest that gingivitis and adult periodontitis subjects with P. gingivalis infection, may recognize different P. gingivalis antigens.

Original languageEnglish (US)
Pages (from-to)63-69
Number of pages7
JournalOral Diseases
Volume1
Issue number2
DOIs
StatePublished - Jun 1995

Fingerprint

Porphyromonas gingivalis
Gingivitis
Periodontitis
Chronic Periodontitis
Antibody Formation
Antigens
Serum
Molecular Weight
Antibodies
Infection
Western Blotting
Membranes

Keywords

  • Antibodies
  • P. gingivalis
  • P. gingivolis infection
  • periodontal disease

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)

Cite this

Antibody responses of Porphyromonas gingivalis infected gingivitis and periodontitis subjects. / Gemmell, E.; Polak, B.; Reinhardt, Richard A; Eccleston, J.; Seymour, G. J.

In: Oral Diseases, Vol. 1, No. 2, 06.1995, p. 63-69.

Research output: Contribution to journalArticle

Gemmell, E. ; Polak, B. ; Reinhardt, Richard A ; Eccleston, J. ; Seymour, G. J. / Antibody responses of Porphyromonas gingivalis infected gingivitis and periodontitis subjects. In: Oral Diseases. 1995 ; Vol. 1, No. 2. pp. 63-69.
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abstract = "Porphyrornonas gingivalis demonstrates a strong association with adult periodontitis although some individuals with the infection do not experience attachment loss. Therefore differences in the immune response to this organism may be of importance to the outcome of the disease. OBJECTIVE: The aim of this study was to determine whether P. gingivalis positive subjects with and without periodontal breakdown, reacted differently to P. gingivolis antigens as assessed by the pattern of serum antibody reactivity. MATERIALS AND METHODS: Two highly defined groups Of subjects were chosen for this study. Both demOnstrated p gingivalis in their plaque and both had responded to P. gingivolis as shown by the presence of serum antibodies* The two groups differed only in their apparent clinical susceptibility to periodontal breakd˜wn. Western blots of P. gingivolis membrane antigens were probed with sera from the two groups to determine their reactivity to specific antigens. RESULTS: Analysis Of the immunoblots showed that there were no differences in either the total numbers of bands, Or bands recognized by the majority Of subjects in the gingivitis and adult periodontitis groups. There were however, four bands recognized by the majority of the gingivitis group and not by the majority Of the adult periodontitis group, there being a significant difference (P = 0.03) in the recognition of the 91.4‐kDa antigen band* A further five antigens Of lower molecular weight were seen by the majority of the adult periodontitis group and not by the majority of the gingivitis group. When sera were tested against purified p*gingivalis Lps, the results indicated that the five antigens seen by the majority Of the adult periodontitis group had molecular weights which were in the range exhibited by the Lps antigens. CONCLUSION: These results suggest that gingivitis and adult periodontitis subjects with P. gingivalis infection, may recognize different P. gingivalis antigens.",
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