Antibody labeling with radioiodine and radiometals

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Antibodies have been conjugated to radionuclides for various in vitro and in vivo applications. Radiolabeled antibodies have been used in clinics and research for diagnostic applications both in vitro as reagents in bioassays and in vivo as imaging agents. Further, radiolabeled antibodies are used as direct therapeutic agents for cancer radioimmunotherapy or as tracers for studying the pharmacokinetics and biodistribution of therapeutic antibodies. Antibodies are labeled with radiohalogens or radiometals, and the choice of candidate radionuclides for a given application is dictated by their emission range and half-life. The conjugation chemistry for the coupling of MAbs with the radiometals requires a chelator, whereas radiohalogens can be incorporated directly in the antibody backbone. In this chapter, we describe the commonly used methods for radiolabeling and characterizing the antibodies most commonly used radiohalogens (125I/131I) and radiometals (177Lu/99mTc).

Original languageEnglish (US)
Title of host publicationDrug Delivery System
PublisherHumana Press Inc.
Pages147-157
Number of pages11
ISBN (Print)9781493903627
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1141
ISSN (Print)1064-3745

Fingerprint

Antibodies
Radioisotopes
Radioimmunotherapy
Chelating Agents
Biological Assay
Half-Life
Pharmacokinetics
Therapeutics
Research
Neoplasms
In Vitro Techniques

Keywords

  • 177Lu
  • 99mTc
  • Antibodies
  • Chelator
  • DOTA
  • DTPA
  • Iodogen
  • Radioiodine
  • Radiolabeling
  • Radiometals
  • Tracers

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Gupta, S., Batra, S. K., & Jain, M. (2014). Antibody labeling with radioiodine and radiometals. In Drug Delivery System (pp. 147-157). (Methods in Molecular Biology; Vol. 1141). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-363-4_9

Antibody labeling with radioiodine and radiometals. / Gupta, Suprit; Batra, Surinder Kumar; Jain, Maneesh.

Drug Delivery System. Humana Press Inc., 2014. p. 147-157 (Methods in Molecular Biology; Vol. 1141).

Research output: Chapter in Book/Report/Conference proceedingChapter

Gupta, S, Batra, SK & Jain, M 2014, Antibody labeling with radioiodine and radiometals. in Drug Delivery System. Methods in Molecular Biology, vol. 1141, Humana Press Inc., pp. 147-157. https://doi.org/10.1007/978-1-4939-363-4_9
Gupta S, Batra SK, Jain M. Antibody labeling with radioiodine and radiometals. In Drug Delivery System. Humana Press Inc. 2014. p. 147-157. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-363-4_9
Gupta, Suprit ; Batra, Surinder Kumar ; Jain, Maneesh. / Antibody labeling with radioiodine and radiometals. Drug Delivery System. Humana Press Inc., 2014. pp. 147-157 (Methods in Molecular Biology).
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