Antibodies to laminin in Chagas' disease

A. Szarfman, V. P. Terranova, Stephen Israel Rennard, J. M. Foidart, M. de Fatima Lima, J. I. Scheinman, G. R. Martin

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained trypomastigotes and mastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite.

Original languageEnglish (US)
Pages (from-to)1161-1171
Number of pages11
JournalJournal of Experimental Medicine
Volume155
Issue number4
DOIs
StatePublished - Jun 1 1982

Fingerprint

Chagas Disease
Laminin
Antibodies
Trypanosoma cruzi
Connective Tissue
Collagen Type III
Heparitin Sulfate
Proteoglycans
Collagen Type I
Macaca mulatta
Serum
Fibronectins
Sepharose
Primates
Immunoglobulin M
Parasites
Immunoglobulin G

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Szarfman, A., Terranova, V. P., Rennard, S. I., Foidart, J. M., de Fatima Lima, M., Scheinman, J. I., & Martin, G. R. (1982). Antibodies to laminin in Chagas' disease. Journal of Experimental Medicine, 155(4), 1161-1171. https://doi.org/10.1084/jem.155.4.1161

Antibodies to laminin in Chagas' disease. / Szarfman, A.; Terranova, V. P.; Rennard, Stephen Israel; Foidart, J. M.; de Fatima Lima, M.; Scheinman, J. I.; Martin, G. R.

In: Journal of Experimental Medicine, Vol. 155, No. 4, 01.06.1982, p. 1161-1171.

Research output: Contribution to journalArticle

Szarfman, A, Terranova, VP, Rennard, SI, Foidart, JM, de Fatima Lima, M, Scheinman, JI & Martin, GR 1982, 'Antibodies to laminin in Chagas' disease', Journal of Experimental Medicine, vol. 155, no. 4, pp. 1161-1171. https://doi.org/10.1084/jem.155.4.1161
Szarfman A, Terranova VP, Rennard SI, Foidart JM, de Fatima Lima M, Scheinman JI et al. Antibodies to laminin in Chagas' disease. Journal of Experimental Medicine. 1982 Jun 1;155(4):1161-1171. https://doi.org/10.1084/jem.155.4.1161
Szarfman, A. ; Terranova, V. P. ; Rennard, Stephen Israel ; Foidart, J. M. ; de Fatima Lima, M. ; Scheinman, J. I. ; Martin, G. R. / Antibodies to laminin in Chagas' disease. In: Journal of Experimental Medicine. 1982 ; Vol. 155, No. 4. pp. 1161-1171.
@article{a1e1b6c252d74b50ba0ca712cd26ba4a,
title = "Antibodies to laminin in Chagas' disease",
abstract = "We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained trypomastigotes and mastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite.",
author = "A. Szarfman and Terranova, {V. P.} and Rennard, {Stephen Israel} and Foidart, {J. M.} and {de Fatima Lima}, M. and Scheinman, {J. I.} and Martin, {G. R.}",
year = "1982",
month = "6",
day = "1",
doi = "10.1084/jem.155.4.1161",
language = "English (US)",
volume = "155",
pages = "1161--1171",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "4",

}

TY - JOUR

T1 - Antibodies to laminin in Chagas' disease

AU - Szarfman, A.

AU - Terranova, V. P.

AU - Rennard, Stephen Israel

AU - Foidart, J. M.

AU - de Fatima Lima, M.

AU - Scheinman, J. I.

AU - Martin, G. R.

PY - 1982/6/1

Y1 - 1982/6/1

N2 - We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained trypomastigotes and mastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite.

AB - We have found that sera from humans with Chagas' disease and Rhesus monkeys infected with Trypanosoma cruzi contain IgM and IgG antibodies, which react with structures in a variety of connective tissues. These antibodies react with laminin but not with various other purified connective tissue components like collagen types I, III, IV, and V, fibronectin, heparan sulfate (BM-1) proteoglycan, or chondronectin. The tissue-reacting antibodies were isolated by absorption to a laminin-Sepharose column. The bound fraction contained all the tissue-reacting antibodies. These antibodies strongly stained trypomastigotes and amastigotes, but weakly stained trypomastigotes and mastigotes, but weakly stained epimastigotes. These studies show that sera from T. cruzi-infected primates contain antilaminin antibodies, which may be produced by those host in response to a laminin-like molecule present in the parasite.

UR - http://www.scopus.com/inward/record.url?scp=0020055333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020055333&partnerID=8YFLogxK

U2 - 10.1084/jem.155.4.1161

DO - 10.1084/jem.155.4.1161

M3 - Article

VL - 155

SP - 1161

EP - 1171

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 4

ER -