Anion modulation of calcium current voltage dependence and amplitude in salamander rods

Wallace B Thoreson, Salvatore L. Stella

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Hofmeister anions were used to investigate the ability of Cl- replacement to produce inhibition and a hyperpolarizing activation shift in L-type Ca2+ currents (I(Ca)) of rod photoreceptors. Inhibition of I(Ca) largely followed the Hofmeister sequence: Cl-=Br-<NO-3<I-<ClO-4 (ClO-4 caused the greatest suppression). Anion-induced hyperpolarizing activation shifts also followed the Hofmeister sequence: Cl-<Br-<NO-3<I-<ClO-4 (ClO-4 caused the largest shift). Agreement with the Hofmeister sequence suggests that these effects are due to anion interactions at the membrane surface. Hofmeister anions also caused similar hyperpolarizing shifts in the voltage dependence of inwardly rectifying cation currents (I(h)) and outward K+ currents (I(K)) consistent with the hypothesis that hyperpolarizing shifts arise from anion effects on membrane surface potential. Sulfate and phosphate inhibited rod I(Ca) and phosphate caused a significant leftward activation shift suggesting these anions are strongly adsorbed to the membrane. Because of the overlap between the physiological voltage range and the lower part of the I(Ca) activation curve, anion effects on amplitude and activation may influence synaptic transmission at the first retinal synapse. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)142-150
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1464
Issue number1
DOIs
StatePublished - Mar 15 2000

Fingerprint

Urodela
Anions
Modulation
Calcium
Electric potential
Chemical activation
Membranes
Phosphates
Retinal Rod Photoreceptor Cells
Synaptic Transmission
Membrane Potentials
Synapses
Sulfates
Cations

Keywords

  • Chloride
  • Hofmeister anion
  • L-Type calcium current
  • Photoreceptor
  • Retina

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Anion modulation of calcium current voltage dependence and amplitude in salamander rods. / Thoreson, Wallace B; Stella, Salvatore L.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1464, No. 1, 15.03.2000, p. 142-150.

Research output: Contribution to journalArticle

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