Angiogenesis and Expression of Vascular Endothelial Growth Factor, Tumour Necrosis Factor-α and Hypoxia Inducible Factor-1α in Canine Renal Cell Carcinoma

J. Y. Yhee, C. H. Yu, J. H. Kim, K. S. Im, N. H. Kim, B. W. Brodersen, Alan R Doster, J. H. Sur

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present study was to determine the distribution and characteristics of microvessels in various histological types of canine renal cell carcinoma (RCC). The study compared microvessel density (MVD) and distribution of blood vessels according to histological type and evaluated the presence of angiogenesis-related proteins. Nine archival samples of canine RCC were studied. MVD was calculated as the mean number of blood vessels per mm2. The diameter of blood vessels was calculated by determining either the length of the long axis of blood vessels (diametermax) or the mean distance from the centre of each blood vessel to the tunica adventia (diametermean). A significant difference in MVD was evident between RCCs and normal kidneys (46.6 ± 28.0 versus 8.4 ± 2.2 microvessels/mm2). Diametermax in canine RCCs (34.1 ± 14.7 μm) was also significantly different from normal canine kidney (23.2 ± 3.4 μm). Vascular endothelial growth factor (VEGF) was expressed by tumour cells and vascular endothelial cells and tumour necrosis factor (TNF)-α expression was observed in vascular endothelial cells in both neoplastic and normal kidney. Although VEGF is involved in angiogenesis and correlates with tumour stage of development, no correlation was found between VEGF expression and MVD. Tumour-associated macrophages expressing TNF-α and hypoxia inducible factor 1α were identified in peritumoural tissue and may play an important role in angiogenesis.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalJournal of Comparative Pathology
Volume147
Issue number2-3
DOIs
StatePublished - Aug 1 2012

Fingerprint

Hypoxia-Inducible Factor 1
vascular endothelial growth factors
tumor necrosis factors
kidney cells
angiogenesis
Microvessels
Renal Cell Carcinoma
blood vessels
Vascular Endothelial Growth Factor A
carcinoma
Canidae
Tumor Necrosis Factor-alpha
Blood Vessels
dogs
Kidney
kidneys
endothelial cells
Endothelial Cells
Neoplasms
neoplasms

Keywords

  • Angiogenesis
  • Dog
  • Microvessel density
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • veterinary(all)

Cite this

Angiogenesis and Expression of Vascular Endothelial Growth Factor, Tumour Necrosis Factor-α and Hypoxia Inducible Factor-1α in Canine Renal Cell Carcinoma. / Yhee, J. Y.; Yu, C. H.; Kim, J. H.; Im, K. S.; Kim, N. H.; Brodersen, B. W.; Doster, Alan R; Sur, J. H.

In: Journal of Comparative Pathology, Vol. 147, No. 2-3, 01.08.2012, p. 129-138.

Research output: Contribution to journalArticle

Yhee, J. Y. ; Yu, C. H. ; Kim, J. H. ; Im, K. S. ; Kim, N. H. ; Brodersen, B. W. ; Doster, Alan R ; Sur, J. H. / Angiogenesis and Expression of Vascular Endothelial Growth Factor, Tumour Necrosis Factor-α and Hypoxia Inducible Factor-1α in Canine Renal Cell Carcinoma. In: Journal of Comparative Pathology. 2012 ; Vol. 147, No. 2-3. pp. 129-138.
@article{a2a134c7d25d44baba73ee62c3c9db1e,
title = "Angiogenesis and Expression of Vascular Endothelial Growth Factor, Tumour Necrosis Factor-α and Hypoxia Inducible Factor-1α in Canine Renal Cell Carcinoma",
abstract = "The aim of the present study was to determine the distribution and characteristics of microvessels in various histological types of canine renal cell carcinoma (RCC). The study compared microvessel density (MVD) and distribution of blood vessels according to histological type and evaluated the presence of angiogenesis-related proteins. Nine archival samples of canine RCC were studied. MVD was calculated as the mean number of blood vessels per mm2. The diameter of blood vessels was calculated by determining either the length of the long axis of blood vessels (diametermax) or the mean distance from the centre of each blood vessel to the tunica adventia (diametermean). A significant difference in MVD was evident between RCCs and normal kidneys (46.6 ± 28.0 versus 8.4 ± 2.2 microvessels/mm2). Diametermax in canine RCCs (34.1 ± 14.7 μm) was also significantly different from normal canine kidney (23.2 ± 3.4 μm). Vascular endothelial growth factor (VEGF) was expressed by tumour cells and vascular endothelial cells and tumour necrosis factor (TNF)-α expression was observed in vascular endothelial cells in both neoplastic and normal kidney. Although VEGF is involved in angiogenesis and correlates with tumour stage of development, no correlation was found between VEGF expression and MVD. Tumour-associated macrophages expressing TNF-α and hypoxia inducible factor 1α were identified in peritumoural tissue and may play an important role in angiogenesis.",
keywords = "Angiogenesis, Dog, Microvessel density, Renal cell carcinoma",
author = "Yhee, {J. Y.} and Yu, {C. H.} and Kim, {J. H.} and Im, {K. S.} and Kim, {N. H.} and Brodersen, {B. W.} and Doster, {Alan R} and Sur, {J. H.}",
year = "2012",
month = "8",
day = "1",
doi = "10.1016/j.jcpa.2011.12.006",
language = "English (US)",
volume = "147",
pages = "129--138",
journal = "Journal of Comparative Pathology",
issn = "0021-9975",
publisher = "W.B. Saunders Ltd",
number = "2-3",

}

TY - JOUR

T1 - Angiogenesis and Expression of Vascular Endothelial Growth Factor, Tumour Necrosis Factor-α and Hypoxia Inducible Factor-1α in Canine Renal Cell Carcinoma

AU - Yhee, J. Y.

AU - Yu, C. H.

AU - Kim, J. H.

AU - Im, K. S.

AU - Kim, N. H.

AU - Brodersen, B. W.

AU - Doster, Alan R

AU - Sur, J. H.

PY - 2012/8/1

Y1 - 2012/8/1

N2 - The aim of the present study was to determine the distribution and characteristics of microvessels in various histological types of canine renal cell carcinoma (RCC). The study compared microvessel density (MVD) and distribution of blood vessels according to histological type and evaluated the presence of angiogenesis-related proteins. Nine archival samples of canine RCC were studied. MVD was calculated as the mean number of blood vessels per mm2. The diameter of blood vessels was calculated by determining either the length of the long axis of blood vessels (diametermax) or the mean distance from the centre of each blood vessel to the tunica adventia (diametermean). A significant difference in MVD was evident between RCCs and normal kidneys (46.6 ± 28.0 versus 8.4 ± 2.2 microvessels/mm2). Diametermax in canine RCCs (34.1 ± 14.7 μm) was also significantly different from normal canine kidney (23.2 ± 3.4 μm). Vascular endothelial growth factor (VEGF) was expressed by tumour cells and vascular endothelial cells and tumour necrosis factor (TNF)-α expression was observed in vascular endothelial cells in both neoplastic and normal kidney. Although VEGF is involved in angiogenesis and correlates with tumour stage of development, no correlation was found between VEGF expression and MVD. Tumour-associated macrophages expressing TNF-α and hypoxia inducible factor 1α were identified in peritumoural tissue and may play an important role in angiogenesis.

AB - The aim of the present study was to determine the distribution and characteristics of microvessels in various histological types of canine renal cell carcinoma (RCC). The study compared microvessel density (MVD) and distribution of blood vessels according to histological type and evaluated the presence of angiogenesis-related proteins. Nine archival samples of canine RCC were studied. MVD was calculated as the mean number of blood vessels per mm2. The diameter of blood vessels was calculated by determining either the length of the long axis of blood vessels (diametermax) or the mean distance from the centre of each blood vessel to the tunica adventia (diametermean). A significant difference in MVD was evident between RCCs and normal kidneys (46.6 ± 28.0 versus 8.4 ± 2.2 microvessels/mm2). Diametermax in canine RCCs (34.1 ± 14.7 μm) was also significantly different from normal canine kidney (23.2 ± 3.4 μm). Vascular endothelial growth factor (VEGF) was expressed by tumour cells and vascular endothelial cells and tumour necrosis factor (TNF)-α expression was observed in vascular endothelial cells in both neoplastic and normal kidney. Although VEGF is involved in angiogenesis and correlates with tumour stage of development, no correlation was found between VEGF expression and MVD. Tumour-associated macrophages expressing TNF-α and hypoxia inducible factor 1α were identified in peritumoural tissue and may play an important role in angiogenesis.

KW - Angiogenesis

KW - Dog

KW - Microvessel density

KW - Renal cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=84864400463&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864400463&partnerID=8YFLogxK

U2 - 10.1016/j.jcpa.2011.12.006

DO - 10.1016/j.jcpa.2011.12.006

M3 - Article

VL - 147

SP - 129

EP - 138

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

SN - 0021-9975

IS - 2-3

ER -