Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA

Ana M. Soto, Janine M. Calabro, Nancy V. Prechtl, Alice Y. Yau, Edward F. Orlando, Andreas Daxenberger, Alan S. Kolok, Louis J. Guillette, Bruno le Bizec, Iris G. Lange, Carlos Sonnenschein

Research output: Contribution to journalArticle

220 Citations (Scopus)

Abstract

Studies reveal that surface waters worldwide are contaminated with hormonally active agents, many released from sewage treatment plants. Another potential source of aquatic hormonal contamination is livestock feedlot effluent. In this study, we assessed whether feedlot effluent contaminates watercourses by measuring a) total androgenic [methyltrienolone (R1881) equivalents] and estrogenic (17β-estradiol equivalents) activity using the A-SCREEN and E-SCREEN bioassays and b) concentrations of anabolic agents via gas chromatography-mass spectroscopy and enzyme-based immunoassays. Water samples were collected over 3 years from up to six sites [all confluent with the Elkhorn River, Nebraska, USA: a feedlot retention pond (site 1), a site downstream from site 1 (site 2), a stream with intermediate livestock impact (site 3), and three sites with no observable livestock impact (sites 4-6)] and two sources of tap water. In 1999, samples from site 1 contained 9.6 pM R1881 equivalents and 1.7 pM 17β-estradiol equivalents. Site 2 samples had estrogen levels similar to those in site 1 samples but lower androgen levels (3.8 pM R1881 equivalents). Androgen levels in site 3 samples were similar to those in site 2 samples, whereas estrogen levels decreased to 0.7 pM 17β-estradiol equivalents. At site 6, androgen levels were approximately half those found at site 3, and estrogen levels were comparable with those at site 3. Sampling in later years was limited to fewer sites because of drought and lack of permission to access one site. Instrumental analysis revealed estrone but no significant levels of resorcylic acid lactones or trenbolone metabolites. Tap water was devoid of hormonal activity. We conclude that feedlot effluents contain sufficient levels of hormonally active agents to warrant further investigation of possible effects on aquatic ecosystem health.

Original languageEnglish (US)
Pages (from-to)346-352
Number of pages7
JournalEnvironmental Health Perspectives
Volume112
Issue number3
StatePublished - Mar 1 2004

Fingerprint

Metribolone
androgen
Body Water
Agriculture
Androgens
cattle
Effluents
livestock
Estradiol
Estrogens
Livestock
effluent
Water
Trenbolone Acetate
Anabolic Agents
Sewage treatment plants
Aquatic ecosystems
Bioassay
Estrone
ecosystem health

Keywords

  • A-SCREEN
  • Acquatic ecosystem health
  • Agricultural runoff
  • Anabolic steroid hormones
  • Concentrated animal feeding operations (CAFOs)
  • E-SCREEN
  • Environmental androgens
  • Personal care products
  • Pharmaceuticals

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Soto, A. M., Calabro, J. M., Prechtl, N. V., Yau, A. Y., Orlando, E. F., Daxenberger, A., ... Sonnenschein, C. (2004). Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA. Environmental Health Perspectives, 112(3), 346-352.

Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA. / Soto, Ana M.; Calabro, Janine M.; Prechtl, Nancy V.; Yau, Alice Y.; Orlando, Edward F.; Daxenberger, Andreas; Kolok, Alan S.; Guillette, Louis J.; le Bizec, Bruno; Lange, Iris G.; Sonnenschein, Carlos.

In: Environmental Health Perspectives, Vol. 112, No. 3, 01.03.2004, p. 346-352.

Research output: Contribution to journalArticle

Soto, AM, Calabro, JM, Prechtl, NV, Yau, AY, Orlando, EF, Daxenberger, A, Kolok, AS, Guillette, LJ, le Bizec, B, Lange, IG & Sonnenschein, C 2004, 'Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA', Environmental Health Perspectives, vol. 112, no. 3, pp. 346-352.
Soto AM, Calabro JM, Prechtl NV, Yau AY, Orlando EF, Daxenberger A et al. Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA. Environmental Health Perspectives. 2004 Mar 1;112(3):346-352.
Soto, Ana M. ; Calabro, Janine M. ; Prechtl, Nancy V. ; Yau, Alice Y. ; Orlando, Edward F. ; Daxenberger, Andreas ; Kolok, Alan S. ; Guillette, Louis J. ; le Bizec, Bruno ; Lange, Iris G. ; Sonnenschein, Carlos. / Androgenic and estrogenic activity in water bodies receiving cattle feedlot effluent in Eastern Nebraska, USA. In: Environmental Health Perspectives. 2004 ; Vol. 112, No. 3. pp. 346-352.
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