Anchoring a Cationic Ligand: The Structure of the Fab Fragment of the Anti-morphine Antibody 9B1 and its Complex with Morphine

Edwin Pozharski, Mark A. Wilson, Anura Hewagama, Armen B. Shanafelt, Gregory Petsko, Dagmar Ringe

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The crystal structures of an anti-morphine antibody 9B1 (to 1.6Å resolution) and its complex with morphine (to 2.0Å resolution) are reported. The morphine-binding site is described as a shallow depression on the protein surface, an unusual topology for a high-affinity (Ka∼109M-1) antibody against a small antigen. The polar part of the ligand is exposed to solvent, and the cationic nitrogen atom of the morphine molecule is anchored at the bottom of the binding site by a salt-bridge to a glutamate side-chain. Additional affinity is provided by a double cation-π interaction with two tryptophan residues. Comparison of the morphine complex with the structure of the free Fab shows that a domain closure occurs upon binding of the ligand.

Original languageEnglish (US)
Pages (from-to)691-697
Number of pages7
JournalJournal of Molecular Biology
Volume337
Issue number3
DOIs
StatePublished - Mar 26 2004

Fingerprint

Immunoglobulin Fab Fragments
Morphine
Anti-Idiotypic Antibodies
Ligands
Binding Sites
Tryptophan
Cations
Glutamic Acid
Membrane Proteins
Nitrogen
Salts
Antigens
Antibodies

Keywords

  • CDR, complementarity determining region
  • Cation-π interaction
  • Domain motion
  • Electrostatics
  • Hapten
  • Protein-ligand interaction

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Anchoring a Cationic Ligand : The Structure of the Fab Fragment of the Anti-morphine Antibody 9B1 and its Complex with Morphine. / Pozharski, Edwin; Wilson, Mark A.; Hewagama, Anura; Shanafelt, Armen B.; Petsko, Gregory; Ringe, Dagmar.

In: Journal of Molecular Biology, Vol. 337, No. 3, 26.03.2004, p. 691-697.

Research output: Contribution to journalArticle

Pozharski, Edwin ; Wilson, Mark A. ; Hewagama, Anura ; Shanafelt, Armen B. ; Petsko, Gregory ; Ringe, Dagmar. / Anchoring a Cationic Ligand : The Structure of the Fab Fragment of the Anti-morphine Antibody 9B1 and its Complex with Morphine. In: Journal of Molecular Biology. 2004 ; Vol. 337, No. 3. pp. 691-697.
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