Analytical methods for kinetic studies of biological interactions: A review

Xiwei Zheng, Cong Bi, Zhao Li, Maria Podariu, David S. Hage

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.

Original languageEnglish (US)
Pages (from-to)163-180
Number of pages18
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume113
DOIs
StatePublished - Sep 1 2015

Fingerprint

Kinetics
Affinity chromatography
Capillary electrophoresis
Surface plasmon resonance
Surface Plasmon Resonance
Capillary Electrophoresis
Affinity Chromatography
Spectroscopy
Spectrum Analysis

Keywords

  • Affinity chromatography
  • Capillary electrophoresis
  • Kinetics of biological interactions
  • Stopped-flow analysis
  • Surface plasmon resonance spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Analytical methods for kinetic studies of biological interactions : A review. / Zheng, Xiwei; Bi, Cong; Li, Zhao; Podariu, Maria; Hage, David S.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 113, 01.09.2015, p. 163-180.

Research output: Contribution to journalReview article

@article{aad8a7ec7a7b4ffa8896dbf393b389c9,
title = "Analytical methods for kinetic studies of biological interactions: A review",
abstract = "The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.",
keywords = "Affinity chromatography, Capillary electrophoresis, Kinetics of biological interactions, Stopped-flow analysis, Surface plasmon resonance spectroscopy",
author = "Xiwei Zheng and Cong Bi and Zhao Li and Maria Podariu and Hage, {David S.}",
year = "2015",
month = "9",
day = "1",
doi = "10.1016/j.jpba.2015.01.042",
language = "English (US)",
volume = "113",
pages = "163--180",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
issn = "0731-7085",
publisher = "Elsevier",

}

TY - JOUR

T1 - Analytical methods for kinetic studies of biological interactions

T2 - A review

AU - Zheng, Xiwei

AU - Bi, Cong

AU - Li, Zhao

AU - Podariu, Maria

AU - Hage, David S.

PY - 2015/9/1

Y1 - 2015/9/1

N2 - The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.

AB - The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.

KW - Affinity chromatography

KW - Capillary electrophoresis

KW - Kinetics of biological interactions

KW - Stopped-flow analysis

KW - Surface plasmon resonance spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=84939255379&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939255379&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2015.01.042

DO - 10.1016/j.jpba.2015.01.042

M3 - Review article

C2 - 25700721

AN - SCOPUS:84939255379

VL - 113

SP - 163

EP - 180

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -