Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants

Guodong Ren, Xiaoyan Wang, Bin Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Uridylation (3′ untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5′ cleavage products (5′ CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5′ CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3′ end uridylation of both AGO-bound miRNAs and 5′ CP.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages23-37
Number of pages15
Volume1640
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1640
ISSN (Print)1064-3745

Fingerprint

Plant RNA
MicroRNAs
RNA Cleavage
Uridine

Keywords

  • 5′ Cleavage product
  • ARGONAUTE
  • HESO1
  • miRNA
  • Rapid amplification of cDNA ends (RACE)
  • Uridylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ren, G., Wang, X., & Yu, B. (2017). Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. In Methods in Molecular Biology (Vol. 1640, pp. 23-37). (Methods in Molecular Biology; Vol. 1640). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7165-7_2

Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. / Ren, Guodong; Wang, Xiaoyan; Yu, Bin.

Methods in Molecular Biology. Vol. 1640 Humana Press Inc., 2017. p. 23-37 (Methods in Molecular Biology; Vol. 1640).

Research output: Chapter in Book/Report/Conference proceedingChapter

Ren, G, Wang, X & Yu, B 2017, Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. in Methods in Molecular Biology. vol. 1640, Methods in Molecular Biology, vol. 1640, Humana Press Inc., pp. 23-37. https://doi.org/10.1007/978-1-4939-7165-7_2
Ren G, Wang X, Yu B. Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. In Methods in Molecular Biology. Vol. 1640. Humana Press Inc. 2017. p. 23-37. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7165-7_2
Ren, Guodong ; Wang, Xiaoyan ; Yu, Bin. / Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. Methods in Molecular Biology. Vol. 1640 Humana Press Inc., 2017. pp. 23-37 (Methods in Molecular Biology).
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