Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants

Guodong Ren, Xiaoyan Wang, Bin Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Uridylation (3′ untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5′ cleavage products (5′ CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5′ CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3′ end uridylation of both AGO-bound miRNAs and 5′ CP.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages23-37
Number of pages15
DOIs
Publication statusPublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1640
ISSN (Print)1064-3745

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Keywords

  • 5′ Cleavage product
  • ARGONAUTE
  • HESO1
  • Rapid amplification of cDNA ends (RACE)
  • Uridylation
  • miRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Ren, G., Wang, X., & Yu, B. (2017). Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. In Methods in Molecular Biology (pp. 23-37). (Methods in Molecular Biology; Vol. 1640). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7165-7_2