Analysis of the phosphoproteome of the multicellular bacterium Streptomyces coelicolor A3(2) by protein/peptide fractionation, phosphopeptide enrichment and high-accuracy mass spectrometry

Jennifer L. Parker, Alexandra M.E. Jones, Liliya Serazetdinova, Gerhard Saalbach, Mervyn J. Bibb, Mike J. Naldrett

Research output: Contribution to journalArticle

Abstract

The serine (Ser)/threonine (Thr)/tyrosine (Tyr) phosphoproteome of exponentially growing Streptomyces coelicolor A3(2) was analysed using the gel-free approaches of preparative IEF for protein fractionation, followed by strong cation exchange peptide fractionation and phosphopeptide enrichment by TiO2 metal oxide affinity chromatography. Phosphopeptides were identified using LC-ESI-LTQ-Orbitrap™ MS. Forty-six novel phosphorylation sites were identified on 40 proteins involved in gene regulation or signalling, central metabolism, protein biosynthesis, membrane transport and cell division, as well as several of unknown function. In contrast to other studies, Thr phosphorylation appeared to be preferred, with relative levels of Ser, Thr and Tyr phosphorylation of 34, 52 and 14%, respectively. Genes for most of the 40 phosphorylated proteins reside in the central "housekeeping" region of the linear S. coelicolor chromosome, suggesting that in general Ser, Thr and Tyr phosphorylation play a role in regulating essential aspects of metabolism in streptomycetes. A greater number of regulators and putative regulators were also identified compared with other bacterial phosphoproteome studies, potentially reflecting the complex heterotrophic and developmental life style of S. coelicolor. This study is the first analysis of the phosphoproteome of a member of this morphologically complex and industrially important group of microorganisms.

Original languageEnglish (US)
Pages (from-to)2486-2497
Number of pages12
JournalProteomics
Volume10
Issue number13
DOIs
StatePublished - Jul 2010

Fingerprint

Streptomyces coelicolor
Phosphopeptides
Phosphorylation
Threonine
Fractionation
Mass spectrometry
Mass Spectrometry
Bacteria
Serine
Tyrosine
Peptides
Metabolism
Proteins
Affinity chromatography
Housekeeping
Biosynthesis
Protein Biosynthesis
Chromosomes
Affinity Chromatography
Gene expression

Keywords

  • MS
  • Microbiology
  • Phosphorylation
  • Streptomyces coelicolor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Analysis of the phosphoproteome of the multicellular bacterium Streptomyces coelicolor A3(2) by protein/peptide fractionation, phosphopeptide enrichment and high-accuracy mass spectrometry. / Parker, Jennifer L.; Jones, Alexandra M.E.; Serazetdinova, Liliya; Saalbach, Gerhard; Bibb, Mervyn J.; Naldrett, Mike J.

In: Proteomics, Vol. 10, No. 13, 07.2010, p. 2486-2497.

Research output: Contribution to journalArticle

Parker, Jennifer L. ; Jones, Alexandra M.E. ; Serazetdinova, Liliya ; Saalbach, Gerhard ; Bibb, Mervyn J. ; Naldrett, Mike J. / Analysis of the phosphoproteome of the multicellular bacterium Streptomyces coelicolor A3(2) by protein/peptide fractionation, phosphopeptide enrichment and high-accuracy mass spectrometry. In: Proteomics. 2010 ; Vol. 10, No. 13. pp. 2486-2497.
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