Analysis of the peptidoglycan of Rickettsia prowazekii

Huiling Pang, H. H. Winkler

Research output: Contribution to journalComment/debate

17 Citations (Scopus)

Abstract

In the present study, peptidoglycan from Rickettsia prowazekii, an obligate intracellular bacterium, was purified. The rickettsial peptidoglycan is like that of gram-negative bacteria; that is, it is sodium dodecyl sulfate insoluble, lysozyme sensitive, and composed of glutamic acid, alanine, and diaminopimelic acid in a molar ratio of 1.0:2.3:1.0. The small amount of lysine found in the peptidoglycan preparation suggests that a peptidoglycan- linked lipoprotein(s) may be present in the rickettsiae. D-Cycloserine, a D- alanine analog which inhibits the biosynthesis of bacterial cell walls, prevented rickettsial growth in mouse L929 cells at a high concentration and altered the morphology of the rickettsiae at a low concentration. These effects were prevented by the addition of D-alanine. This suggests that R. prowazekii contains D-alanine in the peptidoglycan and has D-Ala-D-Ala ligase and alanine racemase activities.

Original languageEnglish (US)
Pages (from-to)923-926
Number of pages4
JournalJournal of bacteriology
Volume176
Issue number3
DOIs
StatePublished - Jan 1 1994

Fingerprint

Rickettsia prowazekii
Peptidoglycan
Alanine
Rickettsia
Alanine Racemase
Diaminopimelic Acid
Cycloserine
Muramidase
Gram-Negative Bacteria
Sodium Dodecyl Sulfate
Cell Wall
Lipoproteins
Lysine
Glutamic Acid
Bacteria
Growth

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Analysis of the peptidoglycan of Rickettsia prowazekii. / Pang, Huiling; Winkler, H. H.

In: Journal of bacteriology, Vol. 176, No. 3, 01.01.1994, p. 923-926.

Research output: Contribution to journalComment/debate

Pang, Huiling ; Winkler, H. H. / Analysis of the peptidoglycan of Rickettsia prowazekii. In: Journal of bacteriology. 1994 ; Vol. 176, No. 3. pp. 923-926.
@article{a4c07ed11bbe43f1b4a3507c0ccd7e90,
title = "Analysis of the peptidoglycan of Rickettsia prowazekii",
abstract = "In the present study, peptidoglycan from Rickettsia prowazekii, an obligate intracellular bacterium, was purified. The rickettsial peptidoglycan is like that of gram-negative bacteria; that is, it is sodium dodecyl sulfate insoluble, lysozyme sensitive, and composed of glutamic acid, alanine, and diaminopimelic acid in a molar ratio of 1.0:2.3:1.0. The small amount of lysine found in the peptidoglycan preparation suggests that a peptidoglycan- linked lipoprotein(s) may be present in the rickettsiae. D-Cycloserine, a D- alanine analog which inhibits the biosynthesis of bacterial cell walls, prevented rickettsial growth in mouse L929 cells at a high concentration and altered the morphology of the rickettsiae at a low concentration. These effects were prevented by the addition of D-alanine. This suggests that R. prowazekii contains D-alanine in the peptidoglycan and has D-Ala-D-Ala ligase and alanine racemase activities.",
author = "Huiling Pang and Winkler, {H. H.}",
year = "1994",
month = "1",
day = "1",
doi = "10.1128/jb.176.3.923-926.1994",
language = "English (US)",
volume = "176",
pages = "923--926",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Analysis of the peptidoglycan of Rickettsia prowazekii

AU - Pang, Huiling

AU - Winkler, H. H.

PY - 1994/1/1

Y1 - 1994/1/1

N2 - In the present study, peptidoglycan from Rickettsia prowazekii, an obligate intracellular bacterium, was purified. The rickettsial peptidoglycan is like that of gram-negative bacteria; that is, it is sodium dodecyl sulfate insoluble, lysozyme sensitive, and composed of glutamic acid, alanine, and diaminopimelic acid in a molar ratio of 1.0:2.3:1.0. The small amount of lysine found in the peptidoglycan preparation suggests that a peptidoglycan- linked lipoprotein(s) may be present in the rickettsiae. D-Cycloserine, a D- alanine analog which inhibits the biosynthesis of bacterial cell walls, prevented rickettsial growth in mouse L929 cells at a high concentration and altered the morphology of the rickettsiae at a low concentration. These effects were prevented by the addition of D-alanine. This suggests that R. prowazekii contains D-alanine in the peptidoglycan and has D-Ala-D-Ala ligase and alanine racemase activities.

AB - In the present study, peptidoglycan from Rickettsia prowazekii, an obligate intracellular bacterium, was purified. The rickettsial peptidoglycan is like that of gram-negative bacteria; that is, it is sodium dodecyl sulfate insoluble, lysozyme sensitive, and composed of glutamic acid, alanine, and diaminopimelic acid in a molar ratio of 1.0:2.3:1.0. The small amount of lysine found in the peptidoglycan preparation suggests that a peptidoglycan- linked lipoprotein(s) may be present in the rickettsiae. D-Cycloserine, a D- alanine analog which inhibits the biosynthesis of bacterial cell walls, prevented rickettsial growth in mouse L929 cells at a high concentration and altered the morphology of the rickettsiae at a low concentration. These effects were prevented by the addition of D-alanine. This suggests that R. prowazekii contains D-alanine in the peptidoglycan and has D-Ala-D-Ala ligase and alanine racemase activities.

UR - http://www.scopus.com/inward/record.url?scp=0028118440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028118440&partnerID=8YFLogxK

U2 - 10.1128/jb.176.3.923-926.1994

DO - 10.1128/jb.176.3.923-926.1994

M3 - Comment/debate

VL - 176

SP - 923

EP - 926

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -