Analysis of Protein Expression and Gene Mutation of c-kit in Colorectal Neuroendocrine Carcinomas

Olaronke Akintola-Ogunremi, John D. Pfeifer, Benjamin R. Tan, Yan Yan, Xiaopei Zhu, John Hart, John R. Goldblum, Lawrence Burgart, Gregory Y. Lauwers, Elizabeth Montgomery, David Lewin, Kay Washington, Mary Bronner, Shu Yuan Xiao, Joel K. Greenson, Laura Lamps, Audrey Lazenby, Hanlin L. Wang

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Primary neuroendocrine carcinomas of the colon are rare but highly aggressive malignancies. The recent observations that c-kit protooncogene, a tyrosine kinase, is overexpressed in a subset of small cell lung cancer and that selective kinase inhibitors block the in vitro growth of small cell lung cancer cell lines prompted us to investigate the expression and mutation status of the c-kit gene in colorectal neuroendocrine carcinomas. Sixty-six cases of primary colorectal neuroendocrine carcinoma were collected from 13 institutions, including 36 small cell carcinomas and 30 moderately differentiated neuroendocrine carcinomas. Immunohistochemical studies using a polyclonal antibody against c-kit protein (CD117) demonstrated a strong and diffuse cytoplasmic staining in 15 cases (23%), which were relatively equally distributed in the small cell and moderately differentiated subgroups. As controls, 25 conventional colorectal adenocarcinomas, 26 colorectal adenomas and 19 colorectal carcinoid tumors were all negative, whereas 15 gastrointestinal stromal tumors were all positive, for kit expression. In contrast to gastrointestinal stromal tumors, kit-overexpressing neuroendocrine carcinomas showed no mutations in the juxtamembrane domain (exon 11) of the c-kit gene as determined by mutational analysis. Kaplan-Meier analysis with the log-rank test revealed that the patients with kit-positive tumors did not differ significantly in survival from those with kit-negative tumors (P = 0.77). These results indicate that c-kit overexpression observed in a subset of colorectal neuroendocrine carcinomas may not be mediated via activating mutations, and does not appear to be an initiating event during tumorigenesis because of lack of c-kit expression in other types of colorectal epithelial neoplasms. More importantly, our observations may have potential therapeutic implications since specific tyrosine kinase inhibitors have shown promise in the management of patients with kit-expressing malignancies.

Original languageEnglish (US)
Pages (from-to)1551-1558
Number of pages8
JournalAmerican Journal of Surgical Pathology
Volume27
Issue number12
DOIs
StatePublished - Dec 1 2003

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Neuroendocrine Carcinoma
Colorectal Neoplasms
Gene Expression
Mutation
Proteins
Gastrointestinal Stromal Tumors
Small Cell Lung Carcinoma
Protein-Tyrosine Kinases
Neoplasms
Proto-Oncogene Proteins c-kit
Small Cell Carcinoma
Glandular and Epithelial Neoplasms
Carcinoid Tumor
Kaplan-Meier Estimate
Adenoma
Genes
Exons
Colon
Carcinogenesis
Adenocarcinoma

Keywords

  • C-kit
  • CD117
  • Colon
  • Neuroendocrine carcinoma
  • Small cell carcinoma

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

Akintola-Ogunremi, O., Pfeifer, J. D., Tan, B. R., Yan, Y., Zhu, X., Hart, J., ... Wang, H. L. (2003). Analysis of Protein Expression and Gene Mutation of c-kit in Colorectal Neuroendocrine Carcinomas. American Journal of Surgical Pathology, 27(12), 1551-1558. https://doi.org/10.1097/00000478-200312000-00008

Analysis of Protein Expression and Gene Mutation of c-kit in Colorectal Neuroendocrine Carcinomas. / Akintola-Ogunremi, Olaronke; Pfeifer, John D.; Tan, Benjamin R.; Yan, Yan; Zhu, Xiaopei; Hart, John; Goldblum, John R.; Burgart, Lawrence; Lauwers, Gregory Y.; Montgomery, Elizabeth; Lewin, David; Washington, Kay; Bronner, Mary; Xiao, Shu Yuan; Greenson, Joel K.; Lamps, Laura; Lazenby, Audrey; Wang, Hanlin L.

In: American Journal of Surgical Pathology, Vol. 27, No. 12, 01.12.2003, p. 1551-1558.

Research output: Contribution to journalArticle

Akintola-Ogunremi, O, Pfeifer, JD, Tan, BR, Yan, Y, Zhu, X, Hart, J, Goldblum, JR, Burgart, L, Lauwers, GY, Montgomery, E, Lewin, D, Washington, K, Bronner, M, Xiao, SY, Greenson, JK, Lamps, L, Lazenby, A & Wang, HL 2003, 'Analysis of Protein Expression and Gene Mutation of c-kit in Colorectal Neuroendocrine Carcinomas', American Journal of Surgical Pathology, vol. 27, no. 12, pp. 1551-1558. https://doi.org/10.1097/00000478-200312000-00008
Akintola-Ogunremi, Olaronke ; Pfeifer, John D. ; Tan, Benjamin R. ; Yan, Yan ; Zhu, Xiaopei ; Hart, John ; Goldblum, John R. ; Burgart, Lawrence ; Lauwers, Gregory Y. ; Montgomery, Elizabeth ; Lewin, David ; Washington, Kay ; Bronner, Mary ; Xiao, Shu Yuan ; Greenson, Joel K. ; Lamps, Laura ; Lazenby, Audrey ; Wang, Hanlin L. / Analysis of Protein Expression and Gene Mutation of c-kit in Colorectal Neuroendocrine Carcinomas. In: American Journal of Surgical Pathology. 2003 ; Vol. 27, No. 12. pp. 1551-1558.
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