Analysis of nicotinic acetylcholine receptor subunits in the cochlea of the mouse

Dennis G. Drescher, Khalid M. Khan, Glenn E. Green, Barbara J Morley, Kirk W. Beisel, Hitesh Kaul, David Gordon, Anil K. Gupta, Marian J. Drescher, Rob L. Barretto

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The present study was designed to catalogue and compare nicotinic receptor subunit messages in the mammalian cochlea. Fourteen nicotinic acetylcholine receptor subunit messages were examined by polymerase chain reaction (PCR) analysis and nucleotide sequencing. Total RNA was extracted from the auditory organs of 14- to 18-day-old CBAJ mice, and mRNA was purified using oligo-dT cellulose. After reverse transcription, resulting cDNA was amplified by PCR with the use of primers specific for the nucleotide sequences representing the following nicotinic receptor subunits: muscle types α1, β1, γ, δ and ε{lunate} and neuronal types α2,3,4,5,6,7 and β2,3,4. cDNA from cochlear tissue corresponding to the muscle-type receptor subunit β1 and to neuronal-type receptor subunits α2,4,5,6 and β2,3 was amplified, whereas cDNA for muscle types α1, γ, δ and ε{lunate} and neuronal types α3,7 and β4 was not. All PCR products were homologous in nucleotide sequence to the corresponding reference cDNAs from which the primers were designed. The current results indicate that nicotinic acetylcholine receptor (nAChR) subunits that are similar or identical to the stated muscle and neuronal types are expressed in the murine cochlea. The presence of messages corresponding to the muscle-type β1 and neuronal-type nAChR subunits may be correlated with the atypical cholinergic response of cochlear hair cells to agonists and antagonists.

Original languageEnglish (US)
Pages (from-to)267-273
Number of pages7
JournalComparative Biochemistry and Physiology. Part C: Comparative
Volume112
Issue number3
DOIs
StatePublished - Jan 1 1995

Fingerprint

Cochlea
Nicotinic Receptors
Muscles
Complementary DNA
Polymerase Chain Reaction
Auditory Hair Cells
Cholinergic Agents
Reverse Transcription
Nucleotides
RNA
Messenger RNA

Keywords

  • Acetylcholine
  • Cochlea
  • Nicotinic receptors
  • Nucleotide sequencing
  • PCR

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Analysis of nicotinic acetylcholine receptor subunits in the cochlea of the mouse. / Drescher, Dennis G.; Khan, Khalid M.; Green, Glenn E.; Morley, Barbara J; Beisel, Kirk W.; Kaul, Hitesh; Gordon, David; Gupta, Anil K.; Drescher, Marian J.; Barretto, Rob L.

In: Comparative Biochemistry and Physiology. Part C: Comparative, Vol. 112, No. 3, 01.01.1995, p. 267-273.

Research output: Contribution to journalArticle

Drescher, DG, Khan, KM, Green, GE, Morley, BJ, Beisel, KW, Kaul, H, Gordon, D, Gupta, AK, Drescher, MJ & Barretto, RL 1995, 'Analysis of nicotinic acetylcholine receptor subunits in the cochlea of the mouse', Comparative Biochemistry and Physiology. Part C: Comparative, vol. 112, no. 3, pp. 267-273. https://doi.org/10.1016/0742-8413(95)02020-9
Drescher, Dennis G. ; Khan, Khalid M. ; Green, Glenn E. ; Morley, Barbara J ; Beisel, Kirk W. ; Kaul, Hitesh ; Gordon, David ; Gupta, Anil K. ; Drescher, Marian J. ; Barretto, Rob L. / Analysis of nicotinic acetylcholine receptor subunits in the cochlea of the mouse. In: Comparative Biochemistry and Physiology. Part C: Comparative. 1995 ; Vol. 112, No. 3. pp. 267-273.
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