Analysis of miRNA Modifications.

Bin Yu, Xuemei Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

After transcription, a large number of cellular RNAs employ modifications to increase their diversity and functional potential. Modifications can occur on the base, ribose, or both, and are important steps in the maturation of many RNAs. Our lab recently showed that plant microRNAs (miRNAs) possess a 2'-O-methyl group on the ribose of the 3' terminal nucleotide, and that this methyl group is added after miRNA/miRNA* formation. One function of this modification is to protect miRNAs from 3' terminal uridylation by an unknown enzymatic activity. It is possible that uridylation of miRNAs triggers their degradation. Here we describe a protocol to purify a specific miRNA in order to determine its molecular mass so that the presence of a modification can be inferred, an in vivo method to detect 3' terminal modification of miRNAs, and an (alpha-32P) dATP incorporation assay to study 3' terminal uridylation of miRNAs.

Original languageEnglish (US)
Pages (from-to)137-148
Number of pages12
JournalMethods in molecular biology (Clifton, N.J.)
Volume592
StatePublished - 2010

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MicroRNAs
Ribose
RNA
Nucleotides

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Analysis of miRNA Modifications. / Yu, Bin; Chen, Xuemei.

In: Methods in molecular biology (Clifton, N.J.), Vol. 592, 2010, p. 137-148.

Research output: Contribution to journalArticle

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