Analysis of Leigh syndrome mutations in the yeast SURF1 homolog reveals a new member of the cytochrome oxidase assembly factor family

Megan Bestwick, Mi Young Jeong, Oleh Khalimonchuk, Hyung Kim, Dennis R. Winge

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18 Citations (Scopus)

Abstract

Three missense SURF1 mutations identified in patients with Leigh syndrome (LS) were evaluated in the yeast homolog Shy1 protein. Introduction of two of the Leigh mutations, F249T and Y344D, in Shy1 failed to significantly attenuate the function of Shy1 in cytochrome c oxidase (CcO) biogenesis as seen with the human mutations. In contrast, a G137E substitution in Shy1 results in a nonfunctional protein conferring a CcO deficiency. The G137E Shy1 mutant phenocopied shy1Δ cells in impaired Cox1 hemylation and low mitochondrial copper. A genetic screen for allele-specific suppressors of the G137E Shy1 mutant revealed Coa2, Cox10, and a novel factor designated Coa4. Coa2 and Cox10 are previously characterized CcO assembly factors. Coa4 is a twin CX9C motif mitochondrial protein localized in the intermembrane space and associated with the inner membrane. Cells lacking Coa4 are depressed in CcO activity but show no impairment in Cox1 maturation or formation of the Shy1-stabilized Cox1 assembly intermediate. To glean insights into the functional role of Coa4 in CcO biogenesis, an unbiased suppressor screen of coa4Δ cells was conducted. Respiratory function of coa4Δ cells was restored by the overexpression of CYC1 encoding cytochrome c. Cyc1 is known to be important at an ill-defined step in the assembly and/or stability of CcO. This new link to Coa4 may begin to further elucidate the role of Cyc1 in CcO biogenesis.

Original languageEnglish (US)
Pages (from-to)4480-4491
Number of pages12
JournalMolecular and cellular biology
Volume30
Issue number18
DOIs
StatePublished - Sep 2010

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Leigh Disease
Electron Transport Complex IV
Yeasts
Mutation
Cytochrome-c Oxidase Deficiency
Mitochondrial Proteins
Missense Mutation
Cytochromes c
Copper
Proteins
Alleles
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Analysis of Leigh syndrome mutations in the yeast SURF1 homolog reveals a new member of the cytochrome oxidase assembly factor family. / Bestwick, Megan; Jeong, Mi Young; Khalimonchuk, Oleh; Kim, Hyung; Winge, Dennis R.

In: Molecular and cellular biology, Vol. 30, No. 18, 09.2010, p. 4480-4491.

Research output: Contribution to journalArticle

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