Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpevirus 1 (RLB106)

C. Jones, T. J. Newby, T. Holt, Alan R Doster, M. Stone, J. Ciacci-Zanella, C. J. Webster, M. W. Jackwood

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Calves were inoculated with the bovine herpes virus 1 (BHV-1) vaccine strain (RLB 106), which is a temperature sensitive mutant. The route of inoculation was intranasal instillation or intramuscular (IM) injection (flank or neck). As a control, five calves were given placebo by IM injection of the neck. Regardless of the infection route, clinical symptoms did not occur. However, BHV-1 neutralizing antibodies were detected after inoculation demonstrating that sero-conversion occurred. At 60 days post-inoculation, dexamethasone was given by IM injection to attempt reactivation of RLB 106. Only those calves inoculated by the intranasal route shed virus leading to an increase in BHV-1 specific antibodies. As expected, viral DNA and the latency related-RNA were detected in trigeminal ganglia (TG) of calves inoculated by the intranasal route. In contrast, viral nucleic acid was not detected in TG of calves inoculated by the IM route or in calves inoculated with placebo. In cervical ganglia or sacral dorsal root ganglia, viral nucleic acid was not consistently detected. This study provides evidence that efficient latency and reactivation does not occur following IM inoculation. Since serum-neutralizing antibodies were detected in all inoculated calves, IM inoculation led to sero-conversion. Copyright (C) 2000 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)3185-3195
Number of pages11
JournalVaccine
Volume18
Issue number27
DOIs
StatePublished - Jul 15 2000

Fingerprint

Intramuscular Injections
vaccination
calves
Viruses
Trigeminal Ganglion
mutants
Temperature
cattle
Neutralizing Antibodies
Nucleic Acids
intramuscular injection
temperature
Neck
Placebos
viruses
Virus Latency
seroconversion
neutralizing antibodies
Viral DNA
Spinal Ganglia

Keywords

  • Bovine herpes virus
  • Latency
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpevirus 1 (RLB106). / Jones, C.; Newby, T. J.; Holt, T.; Doster, Alan R; Stone, M.; Ciacci-Zanella, J.; Webster, C. J.; Jackwood, M. W.

In: Vaccine, Vol. 18, No. 27, 15.07.2000, p. 3185-3195.

Research output: Contribution to journalArticle

Jones, C, Newby, TJ, Holt, T, Doster, AR, Stone, M, Ciacci-Zanella, J, Webster, CJ & Jackwood, MW 2000, 'Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpevirus 1 (RLB106)', Vaccine, vol. 18, no. 27, pp. 3185-3195. https://doi.org/10.1016/S0264-410X(00)00106-7
Jones, C. ; Newby, T. J. ; Holt, T. ; Doster, Alan R ; Stone, M. ; Ciacci-Zanella, J. ; Webster, C. J. ; Jackwood, M. W. / Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpevirus 1 (RLB106). In: Vaccine. 2000 ; Vol. 18, No. 27. pp. 3185-3195.
@article{59d0fbe5b12243b6a9b9853c8fb8cab8,
title = "Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpevirus 1 (RLB106)",
abstract = "Calves were inoculated with the bovine herpes virus 1 (BHV-1) vaccine strain (RLB 106), which is a temperature sensitive mutant. The route of inoculation was intranasal instillation or intramuscular (IM) injection (flank or neck). As a control, five calves were given placebo by IM injection of the neck. Regardless of the infection route, clinical symptoms did not occur. However, BHV-1 neutralizing antibodies were detected after inoculation demonstrating that sero-conversion occurred. At 60 days post-inoculation, dexamethasone was given by IM injection to attempt reactivation of RLB 106. Only those calves inoculated by the intranasal route shed virus leading to an increase in BHV-1 specific antibodies. As expected, viral DNA and the latency related-RNA were detected in trigeminal ganglia (TG) of calves inoculated by the intranasal route. In contrast, viral nucleic acid was not detected in TG of calves inoculated by the IM route or in calves inoculated with placebo. In cervical ganglia or sacral dorsal root ganglia, viral nucleic acid was not consistently detected. This study provides evidence that efficient latency and reactivation does not occur following IM inoculation. Since serum-neutralizing antibodies were detected in all inoculated calves, IM inoculation led to sero-conversion. Copyright (C) 2000 Elsevier Science Ltd.",
keywords = "Bovine herpes virus, Latency, Vaccine",
author = "C. Jones and Newby, {T. J.} and T. Holt and Doster, {Alan R} and M. Stone and J. Ciacci-Zanella and Webster, {C. J.} and Jackwood, {M. W.}",
year = "2000",
month = "7",
day = "15",
doi = "10.1016/S0264-410X(00)00106-7",
language = "English (US)",
volume = "18",
pages = "3185--3195",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "27",

}

TY - JOUR

T1 - Analysis of latency in cattle after inoculation with a temperature sensitive mutant of bovine herpevirus 1 (RLB106)

AU - Jones, C.

AU - Newby, T. J.

AU - Holt, T.

AU - Doster, Alan R

AU - Stone, M.

AU - Ciacci-Zanella, J.

AU - Webster, C. J.

AU - Jackwood, M. W.

PY - 2000/7/15

Y1 - 2000/7/15

N2 - Calves were inoculated with the bovine herpes virus 1 (BHV-1) vaccine strain (RLB 106), which is a temperature sensitive mutant. The route of inoculation was intranasal instillation or intramuscular (IM) injection (flank or neck). As a control, five calves were given placebo by IM injection of the neck. Regardless of the infection route, clinical symptoms did not occur. However, BHV-1 neutralizing antibodies were detected after inoculation demonstrating that sero-conversion occurred. At 60 days post-inoculation, dexamethasone was given by IM injection to attempt reactivation of RLB 106. Only those calves inoculated by the intranasal route shed virus leading to an increase in BHV-1 specific antibodies. As expected, viral DNA and the latency related-RNA were detected in trigeminal ganglia (TG) of calves inoculated by the intranasal route. In contrast, viral nucleic acid was not detected in TG of calves inoculated by the IM route or in calves inoculated with placebo. In cervical ganglia or sacral dorsal root ganglia, viral nucleic acid was not consistently detected. This study provides evidence that efficient latency and reactivation does not occur following IM inoculation. Since serum-neutralizing antibodies were detected in all inoculated calves, IM inoculation led to sero-conversion. Copyright (C) 2000 Elsevier Science Ltd.

AB - Calves were inoculated with the bovine herpes virus 1 (BHV-1) vaccine strain (RLB 106), which is a temperature sensitive mutant. The route of inoculation was intranasal instillation or intramuscular (IM) injection (flank or neck). As a control, five calves were given placebo by IM injection of the neck. Regardless of the infection route, clinical symptoms did not occur. However, BHV-1 neutralizing antibodies were detected after inoculation demonstrating that sero-conversion occurred. At 60 days post-inoculation, dexamethasone was given by IM injection to attempt reactivation of RLB 106. Only those calves inoculated by the intranasal route shed virus leading to an increase in BHV-1 specific antibodies. As expected, viral DNA and the latency related-RNA were detected in trigeminal ganglia (TG) of calves inoculated by the intranasal route. In contrast, viral nucleic acid was not detected in TG of calves inoculated by the IM route or in calves inoculated with placebo. In cervical ganglia or sacral dorsal root ganglia, viral nucleic acid was not consistently detected. This study provides evidence that efficient latency and reactivation does not occur following IM inoculation. Since serum-neutralizing antibodies were detected in all inoculated calves, IM inoculation led to sero-conversion. Copyright (C) 2000 Elsevier Science Ltd.

KW - Bovine herpes virus

KW - Latency

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=0034662390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034662390&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(00)00106-7

DO - 10.1016/S0264-410X(00)00106-7

M3 - Article

VL - 18

SP - 3185

EP - 3195

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 27

ER -