Analysis of glucocerebrosides of Rye (Secale cereale L. cv Puma) leaf and plasma membrane

Edgar B Cahoon, Daniel V. Lynch

Research output: Contribution to journalArticle

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Abstract

Glucocerebrosides of whole rye (Secale cerale L. cv Puma) leaf and plasma membrane were analyzed using gas chromatography-mass spectrometry and gas chromatography following hydrolysis or as intact molecules purified by reverse-phase high performance liquid chromatography. Fatty acids of acid-hydrolyzed leaf and plasma membrane glucocerebrosides consisted of >98 weight percent saturated and monounsaturated 2-hydroxy fatty acids which contained 16 to 26 carbon atoms. The major fatty acids detected were 2-hydroxynervonic acid (24:1h), 2-hydroxylignoceric acid (24:0h), 2-hydroxyerucic acid (22:1h), and 2-hydroxybehenic acid (22:0h). Long-chain bases of alkalinehydrolyzed glucocerebrosides consisted primarily of cis-trans isomers of the trihydroxy base 4-hydroxysphingenine (t18:1) and the dihydroxy base sphingadienine (d18:2) with lesser amounts of 4-hydroxysphinganine (t18:0) and isomers of sphingenine (d 18:1). Intact, underivatized glucocerebroside molecular species of rye leaf and plasma membrane were separated into more than 30 molecular species using reverse-phase HPLC. The molecular species composition of leaf and plasma membrane were quantitatively and qualitatively similar. The major molecular species was 24:1h-t18:1 which constituted nearly 40 weight percent of leaf and plasma membrane extracts. Several other species including 22:1h-t18:1, 24:1h-t18:1 (isomer), 22:0h-t18:1, 24:1h-d18:2, and 24:0h-t18:1 each comprised 4 to 8% of the total. It is anticipated that the high performance liquid chromatography procedure developed in this study to separate intact, underivatized lipid molecular species will be useful in future studies of the physical properties and biosynthesis of plant glucocerebrosides.

Original languageEnglish (US)
Pages (from-to)58-68
Number of pages11
JournalPlant Physiology
Volume95
Issue number1
DOIs
StatePublished - Jan 1 1991

Fingerprint

Puma
Glucosylceramides
Secale cereale
rye
plasma membrane
Cell Membrane
Acids
acids
Fatty Acids
high performance liquid chromatography
High Pressure Liquid Chromatography
leaves
Gas Chromatography-Mass Spectrometry
isomers
Secale
fatty acids
hydroxy fatty acids
Weights and Measures
Hydroxy Acids
reversed-phase liquid chromatography

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Analysis of glucocerebrosides of Rye (Secale cereale L. cv Puma) leaf and plasma membrane. / Cahoon, Edgar B; Lynch, Daniel V.

In: Plant Physiology, Vol. 95, No. 1, 01.01.1991, p. 58-68.

Research output: Contribution to journalArticle

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