Analysis of differentiation-associated proteins in rat bladder carcinogenesis

Kumiko Ogawa, Tung Tien Sun, Samuel Monroe Cohen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Uroplakins are the major integral membrane proteins synthesized in terminally differentiated, superficial urothelial cells. Alteration of cell differentiation during rat urinary bladder carcinogenesis was analyzed immunohistochemically for the expression of uroplakins. Expression of uroplakins was compared in N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT)-, uracil-, sodium saccharin- or sodium ascorbate-induced urothelial simple hyperplasia, papillary-nodular hyperplasia, papilloma and carcinoma. In controls, uroplakins were located only in superficial cells, especially the luminal surface membrane. In FANFT-induced hyperplasia, including simple hyperplasia, intermediate cells also stained and the staining pattern was disorderly and intermittent. In uracil-induced simple hyperplasia, intermediate cells were stained but in an orderly fashion. In sodium saccharin- or sodium ascorbate-induced simple hyperplasia, superficial cells were swollen but alterations were not observed in the staining pattern. In carcinoma induced by FANFT and uracil, uroplakin expression was very disorderly and focal, usually with no expression on surface cells. It appears that disorderly differentiation is an index of bladder malignancy and is an early event in FANFT-induced lesions but a late event in uracil-, sodium saccharin- and sodium ascorbate-induced lesions.

Original languageEnglish (US)
Pages (from-to)961-965
Number of pages5
JournalCarcinogenesis
Volume17
Issue number5
DOIs
StatePublished - May 1 1996

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Uroplakins
FANFT
Hyperplasia
Carcinogenesis
Urinary Bladder
Uracil
Saccharin
Ascorbic Acid
Proteins
Staining and Labeling
Carcinoma
Papilloma
Cell Differentiation
Membrane Proteins
Sodium
Membranes

ASJC Scopus subject areas

  • Cancer Research

Cite this

Analysis of differentiation-associated proteins in rat bladder carcinogenesis. / Ogawa, Kumiko; Sun, Tung Tien; Cohen, Samuel Monroe.

In: Carcinogenesis, Vol. 17, No. 5, 01.05.1996, p. 961-965.

Research output: Contribution to journalArticle

Ogawa, Kumiko ; Sun, Tung Tien ; Cohen, Samuel Monroe. / Analysis of differentiation-associated proteins in rat bladder carcinogenesis. In: Carcinogenesis. 1996 ; Vol. 17, No. 5. pp. 961-965.
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