Analysis of cyclins in trigeminal ganglia of calves infected with bovine herpesvirus-1

M. T. Winkler, L. S. Schang, Alan R Doster, T. Holt, C. Jones

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Following acute infection of cattle with bovine herpesvirus-1 (BHV-1), cyclin expression was examined in trigeminal ganglia (TG). Cyclin A was primarily detected in the nucleus of TG neurons. In contrast, cyclin D1 and cyclin E were detected in the nucleus and cytoplasm of TG neurons. Uninfected or latently infected calves did not express detectable levels of these cyclins in TG neurons. Following dexamethasone-induced reactivation, cyclins D1, E and A were also detected in TG neurons. In situ hybridization of consecutive sections demonstrated that many neurons expressing cyclins contained viral nucleic acid, demonstrating that they were infected. Based on these observations, we hypothesize that BHV-1 infection activates neuronal cyclin expression to enhance productive infection. It is also possible that the stress of neuronal infection or reactivation leads to cyclin expression.

Original languageEnglish (US)
Pages (from-to)2993-2998
Number of pages6
JournalJournal of General Virology
Volume81
Issue number12
DOIs
StatePublished - Jan 1 2000

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Bovine Herpesvirus 1
Trigeminal Ganglion
Cyclins
Neurons
Cyclin A
Cyclin E
Cyclin D1
Infection
Herpesviridae Infections
Nucleic Acids
Dexamethasone
In Situ Hybridization
Cytoplasm

ASJC Scopus subject areas

  • Virology

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Analysis of cyclins in trigeminal ganglia of calves infected with bovine herpesvirus-1. / Winkler, M. T.; Schang, L. S.; Doster, Alan R; Holt, T.; Jones, C.

In: Journal of General Virology, Vol. 81, No. 12, 01.01.2000, p. 2993-2998.

Research output: Contribution to journalArticle

Winkler, M. T. ; Schang, L. S. ; Doster, Alan R ; Holt, T. ; Jones, C. / Analysis of cyclins in trigeminal ganglia of calves infected with bovine herpesvirus-1. In: Journal of General Virology. 2000 ; Vol. 81, No. 12. pp. 2993-2998.
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