Analysis of coagulation changes associated with supraceliac aortic crossclamping using thromboelastography

P. V. Anagnostopoulos, A. D. Shepard, I. I. Pipinos, S. B.K. Raman, P. A. Chaudhry, T. Mishima, H. Morita, G. Suzuki

Research output: Contribution to journalArticle

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Abstract

Introduction. The etiology of the coagulation changes seen with supraceliac (SC) aortic crossclamping (AXC) remains controversial; both primary fabrinolysis and clotting factor consumption have been implicated. The cause of these changes was investigated with thromboelastography (TEG), a test that measures the viscoelastic properties of thrombus to dynamically assess coagulation and fibrinolysis. Methods. Eight pigs underwent SC AXC for 30 min; 5 pigs undergoing 30 min of infrarenal (IR) aortic clamping served as controls. Blood was drawn before AXC, before unclamping, and 5 and 60 min after unclamping. Thromboelastography and standard coagulation tests [prothrombin time (PT), partial thromboplastin time (PTT), fibrinogen, and platelet count] were performed. Measured TEG parameters included fibrinolytic index (a measure of fibrinolysis), r value (a reflection of intrinsic coagulation cascade activity), and the α angle and K values (measures of the speed of solid clot formation). Repeated measures ANOVA and t test were used for statistical analysis. Results. There was no difference in the fibrinolytic index at any time point between the two groups. Increased activity of the intrinsic coagulation cascade during SC clamping was reflected by a lower R value just before unclamping (12.6 ± 3.0 vs 20.0 ± 3.0, P = 0.048) compared to IR AXC. Decreased speed of solid clot formation was noted 5 min after unclamping in the SC group but not the IR group [as defined by an increased K value (ANOVA, P = 0.010) and a decreased angle value (ANOVA, P = 0.005)]. Fibrinogen levels were lower in the SC than in the IR group 5 (P = 0.013) and 60 min after unclamping (P = 0.02), but PT, PTT, and platelets did not differ between the groups at any time points. Conclusions. Thirty minutes of SC AXC does not result in fibrinolysis. There is increased clotting activity during SC clamping followed by decreased speed of clot formation and decreased fibrinogen levels after unclamping. These changes are consistent with clotting factor consumption.

Original languageEnglish (US)
Pages (from-to)52-58
Number of pages7
JournalJournal of Surgical Research
Volume98
Issue number1
DOIs
StatePublished - Jun 1 2001

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Thrombelastography
Fibrinolysis
Constriction
Fibrinogen
Analysis of Variance
Blood Coagulation Factors
Partial Thromboplastin Time
Prothrombin Time
Swine
Platelet Count
Thrombosis
Blood Platelets

ASJC Scopus subject areas

  • Surgery

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Anagnostopoulos, P. V., Shepard, A. D., Pipinos, I. I., Raman, S. B. K., Chaudhry, P. A., Mishima, T., ... Suzuki, G. (2001). Analysis of coagulation changes associated with supraceliac aortic crossclamping using thromboelastography. Journal of Surgical Research, 98(1), 52-58. https://doi.org/10.1006/jsre.2001.6162

Analysis of coagulation changes associated with supraceliac aortic crossclamping using thromboelastography. / Anagnostopoulos, P. V.; Shepard, A. D.; Pipinos, I. I.; Raman, S. B.K.; Chaudhry, P. A.; Mishima, T.; Morita, H.; Suzuki, G.

In: Journal of Surgical Research, Vol. 98, No. 1, 01.06.2001, p. 52-58.

Research output: Contribution to journalArticle

Anagnostopoulos, PV, Shepard, AD, Pipinos, II, Raman, SBK, Chaudhry, PA, Mishima, T, Morita, H & Suzuki, G 2001, 'Analysis of coagulation changes associated with supraceliac aortic crossclamping using thromboelastography', Journal of Surgical Research, vol. 98, no. 1, pp. 52-58. https://doi.org/10.1006/jsre.2001.6162
Anagnostopoulos, P. V. ; Shepard, A. D. ; Pipinos, I. I. ; Raman, S. B.K. ; Chaudhry, P. A. ; Mishima, T. ; Morita, H. ; Suzuki, G. / Analysis of coagulation changes associated with supraceliac aortic crossclamping using thromboelastography. In: Journal of Surgical Research. 2001 ; Vol. 98, No. 1. pp. 52-58.
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abstract = "Introduction. The etiology of the coagulation changes seen with supraceliac (SC) aortic crossclamping (AXC) remains controversial; both primary fabrinolysis and clotting factor consumption have been implicated. The cause of these changes was investigated with thromboelastography (TEG), a test that measures the viscoelastic properties of thrombus to dynamically assess coagulation and fibrinolysis. Methods. Eight pigs underwent SC AXC for 30 min; 5 pigs undergoing 30 min of infrarenal (IR) aortic clamping served as controls. Blood was drawn before AXC, before unclamping, and 5 and 60 min after unclamping. Thromboelastography and standard coagulation tests [prothrombin time (PT), partial thromboplastin time (PTT), fibrinogen, and platelet count] were performed. Measured TEG parameters included fibrinolytic index (a measure of fibrinolysis), r value (a reflection of intrinsic coagulation cascade activity), and the α angle and K values (measures of the speed of solid clot formation). Repeated measures ANOVA and t test were used for statistical analysis. Results. There was no difference in the fibrinolytic index at any time point between the two groups. Increased activity of the intrinsic coagulation cascade during SC clamping was reflected by a lower R value just before unclamping (12.6 ± 3.0 vs 20.0 ± 3.0, P = 0.048) compared to IR AXC. Decreased speed of solid clot formation was noted 5 min after unclamping in the SC group but not the IR group [as defined by an increased K value (ANOVA, P = 0.010) and a decreased angle value (ANOVA, P = 0.005)]. Fibrinogen levels were lower in the SC than in the IR group 5 (P = 0.013) and 60 min after unclamping (P = 0.02), but PT, PTT, and platelets did not differ between the groups at any time points. Conclusions. Thirty minutes of SC AXC does not result in fibrinolysis. There is increased clotting activity during SC clamping followed by decreased speed of clot formation and decreased fibrinogen levels after unclamping. These changes are consistent with clotting factor consumption.",
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T1 - Analysis of coagulation changes associated with supraceliac aortic crossclamping using thromboelastography

AU - Anagnostopoulos, P. V.

AU - Shepard, A. D.

AU - Pipinos, I. I.

AU - Raman, S. B.K.

AU - Chaudhry, P. A.

AU - Mishima, T.

AU - Morita, H.

AU - Suzuki, G.

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Introduction. The etiology of the coagulation changes seen with supraceliac (SC) aortic crossclamping (AXC) remains controversial; both primary fabrinolysis and clotting factor consumption have been implicated. The cause of these changes was investigated with thromboelastography (TEG), a test that measures the viscoelastic properties of thrombus to dynamically assess coagulation and fibrinolysis. Methods. Eight pigs underwent SC AXC for 30 min; 5 pigs undergoing 30 min of infrarenal (IR) aortic clamping served as controls. Blood was drawn before AXC, before unclamping, and 5 and 60 min after unclamping. Thromboelastography and standard coagulation tests [prothrombin time (PT), partial thromboplastin time (PTT), fibrinogen, and platelet count] were performed. Measured TEG parameters included fibrinolytic index (a measure of fibrinolysis), r value (a reflection of intrinsic coagulation cascade activity), and the α angle and K values (measures of the speed of solid clot formation). Repeated measures ANOVA and t test were used for statistical analysis. Results. There was no difference in the fibrinolytic index at any time point between the two groups. Increased activity of the intrinsic coagulation cascade during SC clamping was reflected by a lower R value just before unclamping (12.6 ± 3.0 vs 20.0 ± 3.0, P = 0.048) compared to IR AXC. Decreased speed of solid clot formation was noted 5 min after unclamping in the SC group but not the IR group [as defined by an increased K value (ANOVA, P = 0.010) and a decreased angle value (ANOVA, P = 0.005)]. Fibrinogen levels were lower in the SC than in the IR group 5 (P = 0.013) and 60 min after unclamping (P = 0.02), but PT, PTT, and platelets did not differ between the groups at any time points. Conclusions. Thirty minutes of SC AXC does not result in fibrinolysis. There is increased clotting activity during SC clamping followed by decreased speed of clot formation and decreased fibrinogen levels after unclamping. These changes are consistent with clotting factor consumption.

AB - Introduction. The etiology of the coagulation changes seen with supraceliac (SC) aortic crossclamping (AXC) remains controversial; both primary fabrinolysis and clotting factor consumption have been implicated. The cause of these changes was investigated with thromboelastography (TEG), a test that measures the viscoelastic properties of thrombus to dynamically assess coagulation and fibrinolysis. Methods. Eight pigs underwent SC AXC for 30 min; 5 pigs undergoing 30 min of infrarenal (IR) aortic clamping served as controls. Blood was drawn before AXC, before unclamping, and 5 and 60 min after unclamping. Thromboelastography and standard coagulation tests [prothrombin time (PT), partial thromboplastin time (PTT), fibrinogen, and platelet count] were performed. Measured TEG parameters included fibrinolytic index (a measure of fibrinolysis), r value (a reflection of intrinsic coagulation cascade activity), and the α angle and K values (measures of the speed of solid clot formation). Repeated measures ANOVA and t test were used for statistical analysis. Results. There was no difference in the fibrinolytic index at any time point between the two groups. Increased activity of the intrinsic coagulation cascade during SC clamping was reflected by a lower R value just before unclamping (12.6 ± 3.0 vs 20.0 ± 3.0, P = 0.048) compared to IR AXC. Decreased speed of solid clot formation was noted 5 min after unclamping in the SC group but not the IR group [as defined by an increased K value (ANOVA, P = 0.010) and a decreased angle value (ANOVA, P = 0.005)]. Fibrinogen levels were lower in the SC than in the IR group 5 (P = 0.013) and 60 min after unclamping (P = 0.02), but PT, PTT, and platelets did not differ between the groups at any time points. Conclusions. Thirty minutes of SC AXC does not result in fibrinolysis. There is increased clotting activity during SC clamping followed by decreased speed of clot formation and decreased fibrinogen levels after unclamping. These changes are consistent with clotting factor consumption.

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