Analysis of bovine trigeminal ganglia following infection with bovine herpesvirus 1

M. T C Winkler, Alan R Doster, J. H. Sur, C. Jones

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Following primary infection of the eye, oral cavity, and/or nasal cavity, bovine herpesvirus 1 (BHV-1) establishes latency in trigeminal ganglionic (TG) neurons. Virus reactivation and spread to other susceptible animals occur after natural or corticosteroid-induced stress. Infection of calves with BHV-1 leads to infiltration of lymphocytes in TG and expression of IFN-γ (interferon-gamma), even in latently infected calves. During latency, virus antigen and nucleic acid positive non-neural cells were occasionally detected in TG suggesting there is a low level of spontaneous reactivation. Since we could not detect virus in ocular or nasal swabs, these rare cells do not support high levels of productive infection and virus release or they do not support virus production at all. Dexamethasone (DEX) was used to initiate reactivation in latently infected calves. Foci of mononuclear or satellite cells undergoing apoptosis were detected 6 h after DEX treatment, as judged by the appearance of TUNEL+ cells (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling). BHV-1 antigen expression was initially detected in lymphocytes and other non-neural cells in latently infected calves following DEX treatment. At 24 h after DEX treatment, viral antigen expression and nucleic acid were readily detected in neurons. Our data suggest that persistent lymphocyte infiltration and cytokine expression occur during latency because a low number of cells in TG express BHV-1 proteins. Induction of apoptosis and changes in cytokine expression following DEX treatment correlates with reactivation from latency. We hypothesize that inflammatory infiltration of lymphoid cells in TG plays a role in regulating latency.

Original languageEnglish (US)
Pages (from-to)139-155
Number of pages17
JournalVeterinary Microbiology
Volume86
Issue number1-2
DOIs
StatePublished - Apr 22 2002

Fingerprint

Bovine Herpesvirus 1
Trigeminal Ganglion
Bovine herpesvirus 1
Dexamethasone
dexamethasone
Lymphocytes
cattle
Viruses
Infection
infection
viruses
calves
Nucleic Acids
cells
lymphocytes
Apoptosis
Cytokines
Virus Release
nucleic acids
Eye Infections

Keywords

  • Apoptosis
  • Bovine herpesvirus type 1
  • Latency
  • Neuroimmunology

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Analysis of bovine trigeminal ganglia following infection with bovine herpesvirus 1. / Winkler, M. T C; Doster, Alan R; Sur, J. H.; Jones, C.

In: Veterinary Microbiology, Vol. 86, No. 1-2, 22.04.2002, p. 139-155.

Research output: Contribution to journalArticle

Winkler, M. T C ; Doster, Alan R ; Sur, J. H. ; Jones, C. / Analysis of bovine trigeminal ganglia following infection with bovine herpesvirus 1. In: Veterinary Microbiology. 2002 ; Vol. 86, No. 1-2. pp. 139-155.
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