Analysis of bovine herpesvirus 1 transcripts during a primary infection of trigeminal ganglia of cattle

Luis M. Schang, Clinton Jones

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

During an infection of nonneuronal cells, bovine herpesvirus 1 (BHV-1) gene expression proceeds in a well-defined cascade. Products of immediate- early (IE) genes are expressed first, and they activate, expression of early (E) and late (L) genes. Although the same cascade is assumed to occur during an infection of neurons in trigeminal ganglia (TG) of cattle, no experimental data is available to support this hypothesis. Consequently, we analyzed BHV- 1 gene expression in bovine TG at 1, 2, 4, 7, and 15 days postinfection (dpi). Infectious virus was detected in ocular swabs from 1 to 7 dpi but not 15 dpi. By reverse transcription (RT)-PCR, IE (bICP4), E (thymidine kinase, ribonucleotide reductase [RR]), L (glycoprotein C, and α trans-inducing factor), and dual-kinetic (bICP0 and bICP22) transcripts were analyzed. When cDNA synthesis was primed with random hexamers, IE and E transcripts were detected at the same time. However, full-length and poly(A)+ (FL and P) RR or bICP22 RNAs were detected before FL and P IE RNAs. Furthermore, FL and P IE transcripts were not detected until viral DNA increased in TG. IE transcripts were detected before E or L RNAs when rabbit kidney cells were infected with a low multiplicity of infection and the same RT-PCR detection method was used. These studies suggested that expression of full-length and polyadenylated IE transcripts in trigeminal ganglia was not efficient compared to that of RR and bICP22 transcripts.

Original languageEnglish (US)
Pages (from-to)6786-6795
Number of pages10
JournalJournal of virology
Volume71
Issue number9
StatePublished - Sep 4 1997

Fingerprint

Bovine Herpesvirus 1
Trigeminal Ganglion
Bovine herpesvirus 1
ribonucleotide reductase
Ribonucleotide Reductases
cattle
RNA
Infection
infection
Reverse Transcription
reverse transcriptase polymerase chain reaction
thymidine kinase
Gene Expression
Polymerase Chain Reaction
gene expression
Poly A
Immediate-Early Genes
Thymidine Kinase
Viral DNA
kidney cells

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Analysis of bovine herpesvirus 1 transcripts during a primary infection of trigeminal ganglia of cattle. / Schang, Luis M.; Jones, Clinton.

In: Journal of virology, Vol. 71, No. 9, 04.09.1997, p. 6786-6795.

Research output: Contribution to journalArticle

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abstract = "During an infection of nonneuronal cells, bovine herpesvirus 1 (BHV-1) gene expression proceeds in a well-defined cascade. Products of immediate- early (IE) genes are expressed first, and they activate, expression of early (E) and late (L) genes. Although the same cascade is assumed to occur during an infection of neurons in trigeminal ganglia (TG) of cattle, no experimental data is available to support this hypothesis. Consequently, we analyzed BHV- 1 gene expression in bovine TG at 1, 2, 4, 7, and 15 days postinfection (dpi). Infectious virus was detected in ocular swabs from 1 to 7 dpi but not 15 dpi. By reverse transcription (RT)-PCR, IE (bICP4), E (thymidine kinase, ribonucleotide reductase [RR]), L (glycoprotein C, and α trans-inducing factor), and dual-kinetic (bICP0 and bICP22) transcripts were analyzed. When cDNA synthesis was primed with random hexamers, IE and E transcripts were detected at the same time. However, full-length and poly(A)+ (FL and P) RR or bICP22 RNAs were detected before FL and P IE RNAs. Furthermore, FL and P IE transcripts were not detected until viral DNA increased in TG. IE transcripts were detected before E or L RNAs when rabbit kidney cells were infected with a low multiplicity of infection and the same RT-PCR detection method was used. These studies suggested that expression of full-length and polyadenylated IE transcripts in trigeminal ganglia was not efficient compared to that of RR and bICP22 transcripts.",
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