Analysis of Bisulfite Via a Nitro Derivative of Cyanine-3 (NCy3) in the Microfluidic Channel

Haley A. Houtwed, Meng Xie, Aatiya Ahmad, Cody D. Masters, Melissa M. Davison, Kristy Kounovsky-Shafer, Haishi Cao

Research output: Contribution to journalArticle


NCy3, a derivative of Cyanine 3 with a nitro substituent, showed a high reactivity to bisulfite in aqueous media, instantly leading to ratiometric change of absorption spectra and significant fluorescence quenching. Applied in the microfluidic channel, NCy3 functionalize as a sensitive approach for quantitative detection of bisulfite, particularly for samples with a small volume.

Original languageEnglish (US)
Pages (from-to)523-529
Number of pages7
JournalJournal of Fluorescence
Issue number3
Publication statusPublished - May 15 2019



  • Bisulfite sensor
  • Cyanine dye
  • Microfluidic channel
  • Nitration

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law

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