Analysis of 94 kb of the Chlorella virus PBCV-1 330-kb genome: Map positions 88 to 182

Zhiqiang Lu, Yu Li, Quideng Que, Gerald F. Kutish, Daniel L. Rock, James L. Van Etten

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Analysis of 94 kb of DNA, located between map positions 88 and 182 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 195 open reading frames (ORFs) 65 codons or longer. One hundred and five of the 195 ORFs were considered major ORFs. Twenty-six of the 105 major ORFs resembled genes in the databases including three chitinases, a chitosanase, three serine/threonine protein kinases, two additional protein kinases, a tyrosine protein phosphatase, two ankyrins, an ornithine decarboxylase, a copper/zinc-superoxide dismutase, a proliferating cell nuclear antigen, a DNA polymerase, a fibronectin-binding protein, the yeast Ski2 protein, an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease, and an amidase. The genes for the 105 major ORFs were evenly distributed along the genome and, except for one noncoding 1788-nucleotide stretch, the genes were close together. Unexpectedly, a 900-bp region in the 1788-bp noncoding sequence resembled a CpG island.

Original languageEnglish (US)
Pages (from-to)102-123
Number of pages22
JournalVirology
Volume216
Issue number1
DOIs
StatePublished - Feb 1 1996

Fingerprint

Chlorella
Open Reading Frames
Genome
Viruses
chitosanase
amidase
DNA
Ankyrins
Genes
Chitinases
CpG Islands
Protein Tyrosine Phosphatases
Ornithine Decarboxylase
Fungal Proteins
Endonucleases
Protein-Serine-Threonine Kinases
Proliferating Cell Nuclear Antigen
Methyltransferases
Adenine
DNA-Directed DNA Polymerase

ASJC Scopus subject areas

  • Virology

Cite this

Analysis of 94 kb of the Chlorella virus PBCV-1 330-kb genome : Map positions 88 to 182. / Lu, Zhiqiang; Li, Yu; Que, Quideng; Kutish, Gerald F.; Rock, Daniel L.; Van Etten, James L.

In: Virology, Vol. 216, No. 1, 01.02.1996, p. 102-123.

Research output: Contribution to journalArticle

Lu, Zhiqiang ; Li, Yu ; Que, Quideng ; Kutish, Gerald F. ; Rock, Daniel L. ; Van Etten, James L. / Analysis of 94 kb of the Chlorella virus PBCV-1 330-kb genome : Map positions 88 to 182. In: Virology. 1996 ; Vol. 216, No. 1. pp. 102-123.
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