Analysis of 74 kb of DNA located at the right end of the 330-kb chlorella virus PBCV-1 genome

Yu Li, Zhiqiang Lu, Liangwu Sun, Susan Ropp, Gerald F. Kutish, Daniel L. Rock, James L. Van Etten

Research output: Contribution to journalArticle

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Abstract

This report completes a preliminary analysis of the sequence of the 330,740-bp chlorella virus PBCV-1 genome, the largest virus genome to be sequenced to date. The PBCV-1 genome is 57% the size of the genome from the smallest self-replicating organism, Mycoplasma genitalium. Analysis of 74 kb of newly sequenced DNA, from the right terminus of the PBCV-1 genome, revealed 153 open reading frames (ORFs) of 65 codons or longer. Eighty-five of these ORFs, which are evenly distributed on both strands of the DNA, were considered major ORFs. Fifty-nine of the major ORFs were separated by less than 100 bp. The largest intergenic distance was 729 bp, which occurred between two ORFs located in the 2.2-kb inverted terminal repeat region of the PBCV-1 genome. Twenty-seven of the 85 major ORFs resemble proteins in databases, including the large subunit of ribonucleotide diphosphate reductase, ATP-dependent DNA ligase, type II DNA topoisomerase, a helicase, histidine decarboxylase, dCMP deaminase, dUTP pyrophosphatase, proliferating cell nuclear antigen, a transposase, fungal translation elongation factor 3 (EF-3), UDP glucose dehydrogenase, a protein kinase, and an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease. Seventeen of the 153 ORFs resembled other PBCV-1 ORFs, suggesting that they represent either gene duplications or gene families.

Original languageEnglish (US)
Pages (from-to)360-377
Number of pages18
JournalVirology
Volume237
Issue number2
DOIs
StatePublished - Oct 27 1997

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Chlorella
Open Reading Frames
Genome
Viruses
DNA
Uridine Diphosphate Glucose Dehydrogenase
Mycoplasma genitalium
Histidine Decarboxylase
Transposases
Peptide Elongation Factors
Ribonucleotide Reductases
Genome Size
Type II DNA Topoisomerase
Protein Databases
Gene Duplication
Terminal Repeat Sequences
Diphosphates
Endonucleases
Proliferating Cell Nuclear Antigen
Methyltransferases

ASJC Scopus subject areas

  • Virology

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Analysis of 74 kb of DNA located at the right end of the 330-kb chlorella virus PBCV-1 genome. / Li, Yu; Lu, Zhiqiang; Sun, Liangwu; Ropp, Susan; Kutish, Gerald F.; Rock, Daniel L.; Van Etten, James L.

In: Virology, Vol. 237, No. 2, 27.10.1997, p. 360-377.

Research output: Contribution to journalArticle

Li, Yu ; Lu, Zhiqiang ; Sun, Liangwu ; Ropp, Susan ; Kutish, Gerald F. ; Rock, Daniel L. ; Van Etten, James L. / Analysis of 74 kb of DNA located at the right end of the 330-kb chlorella virus PBCV-1 genome. In: Virology. 1997 ; Vol. 237, No. 2. pp. 360-377.
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