Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genorne

Zhiqiang Lu, Yu Li, Yanping Zhang, Gerald F. Kutish, Daniel L. Rock, James L. Van Etten

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Forty-five kilobases of DNA, including the previously sequenced 2.2-kb inverted repeat region, located at the left terminiof the 330-kb Chlorella virus PBCV-1 genome were sequenced and analyzed. Eighty-five complete open reading frames (ORFs) larger than 195 nucleotides were identified. Thirty-seven of the 85 ORFs, which are densely packed on both strands of the DNA, were considered major ORFs. Fifteen of the major ORFs have similarity to genes in the databases, including bacterial glycerophosphoryl diester phosphodiesterase, bacteriophage T4 endonuclease V, d-isomer specific 2-hydroxyacid dehydrogenases, and β-alanine synthetase and bacterial nitrilases. Two major ORFs resemble the virus major capsid protein. Three major ORFs contain three or more ankyrin-like repeat elements and four ORFs encode proline-rich proteins.

Original languageEnglish (US)
Pages (from-to)339-352
Number of pages14
JournalVirology
Volume206
Issue number1
DOIs
StatePublished - Jan 10 1995

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Chlorella
Open Reading Frames
Viruses
DNA
nitrilase
Ankyrin Repeat
Phosphoric Diester Hydrolases
Capsid Proteins
Ligases
Proline
Alanine
Nucleotides
Genome
Databases

ASJC Scopus subject areas

  • Virology

Cite this

Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genorne. / Lu, Zhiqiang; Li, Yu; Zhang, Yanping; Kutish, Gerald F.; Rock, Daniel L.; Van Etten, James L.

In: Virology, Vol. 206, No. 1, 10.01.1995, p. 339-352.

Research output: Contribution to journalArticle

Lu, Zhiqiang ; Li, Yu ; Zhang, Yanping ; Kutish, Gerald F. ; Rock, Daniel L. ; Van Etten, James L. / Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genorne. In: Virology. 1995 ; Vol. 206, No. 1. pp. 339-352.
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