An oxidative environment promotes growth of Mycobacterium abscessus

Rebecca E. Oberley-Deegan, Brittany W. Rebits, Michael R. Weaver, Angela K. Tollefson, Xiyuan Bai, Mischa McGibney, Alida R. Ovrutsky, Edward D. Chan, James D. Crapo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Mycobacterium abscessus infections, particularly those causing chronic lung diseases, are becoming more prevalent worldwide. M. abscessus infections are difficult to treat because of antibiotic resistance. Thus, new treatment options are urgently needed. M. abscessus is an intracellular pathogen that primarily infects macrophages and fibroblasts. Because this bacterium has only recently been identified as a separate species, very little is known about M. abscessus-host interactions and how M. abscessus growth is regulated. Oxidative stress has long been shown to inhibit the growth of bacterial organisms. However, some intracellular bacteria, such as Mycobacterium tuberculosis, grow well in oxidizing environments. In this study, we show that M. abscessus infection causes the host cell environment to become more oxidizing. Furthermore, we show that a more oxidizing environment leads to enhanced growth of M. abscessus inside macrophages. In the presence of antioxidants, MnTE-2-PyP (chemical name: manganese(II) meso-tetrakis-(N-methylpyridinium-2-yl) porphyrin) or N-acetyl-l-cysteine, M. abscessus growth is inhibited. These results lead us to postulate that antioxidants may aid in the treatment of M. abscessus infections.

Original languageEnglish (US)
Pages (from-to)1666-1673
Number of pages8
JournalFree Radical Biology and Medicine
Volume49
Issue number11
DOIs
StatePublished - Dec 1 2010

Fingerprint

Mycobacterium
Macrophages
Growth
Bacteria
Antioxidants
Infection
Acetylcysteine
Pulmonary diseases
Mycobacterium Infections
Oxidative stress
Porphyrins
Pathogens
Fibroblasts
Manganese
Microbial Drug Resistance
Mycobacterium tuberculosis
Lung Diseases
Names
Cysteine
Oxidative Stress

Keywords

  • Alveolar macrophages
  • Antioxidants
  • Free radicals
  • MnTE-2-PyP
  • Mycobacterium abscessus
  • N-acetyl-l-cysteine
  • Oxidation
  • Smoking
  • THP-1

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Oberley-Deegan, R. E., Rebits, B. W., Weaver, M. R., Tollefson, A. K., Bai, X., McGibney, M., ... Crapo, J. D. (2010). An oxidative environment promotes growth of Mycobacterium abscessus. Free Radical Biology and Medicine, 49(11), 1666-1673. https://doi.org/10.1016/j.freeradbiomed.2010.08.026

An oxidative environment promotes growth of Mycobacterium abscessus. / Oberley-Deegan, Rebecca E.; Rebits, Brittany W.; Weaver, Michael R.; Tollefson, Angela K.; Bai, Xiyuan; McGibney, Mischa; Ovrutsky, Alida R.; Chan, Edward D.; Crapo, James D.

In: Free Radical Biology and Medicine, Vol. 49, No. 11, 01.12.2010, p. 1666-1673.

Research output: Contribution to journalArticle

Oberley-Deegan, RE, Rebits, BW, Weaver, MR, Tollefson, AK, Bai, X, McGibney, M, Ovrutsky, AR, Chan, ED & Crapo, JD 2010, 'An oxidative environment promotes growth of Mycobacterium abscessus', Free Radical Biology and Medicine, vol. 49, no. 11, pp. 1666-1673. https://doi.org/10.1016/j.freeradbiomed.2010.08.026
Oberley-Deegan, Rebecca E. ; Rebits, Brittany W. ; Weaver, Michael R. ; Tollefson, Angela K. ; Bai, Xiyuan ; McGibney, Mischa ; Ovrutsky, Alida R. ; Chan, Edward D. ; Crapo, James D. / An oxidative environment promotes growth of Mycobacterium abscessus. In: Free Radical Biology and Medicine. 2010 ; Vol. 49, No. 11. pp. 1666-1673.
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