An orthotopic mouse model of remetastasis of human colon cancer liver metastasis

Babak Rashidi, Reza Gamagami, Aaron R Sasson, Fang Xian Sun, Jack Geller, A. R. Moossa, Robert M. Hoffman

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Whether liver metastases from colon cancer are capable of metastasizing to other sites is an important question in surgical oncology. To answer this question, we have developed a highly metastatic orthotopic transplant model of a liver metastasis from a human colon cancer patient in nude mice that targets the liver and lymph nodes. The metastatic human tumor was transplanted in athymic nude mice by surgical orthotopic implantation (SOI) of a liver metastasis from a colon cancer patient. The human colon tumor was then subsequently implanted in the colon by SOI or, in an additional series of nude mice, in the liver by surgical hepatic implantation (SHI). The mice were then explored over time for lymph node involvement beginning 10 days after implantation. After SOI, 100% of the animals had liver metastasis within 10 days, and subsequently, 19 days after SOI, all lymph nodes draining the liver were involved with metastasis without any retroperitoneal or lung tissue involvement. After SHI, all sites of lymphatic drainage of the liver, including portal, celiac, and mediastinal lymph nodes, were massively involved by metastasis in 100% of the animals as early as 10 days after tumor implantation on the liver. The results of this study demonstrate that liver metastases from colon cancer are capable of remetastasizing to other sites. This study thus suggests that in colon cancer patients with liver metastasis, mediastinal, celiac, and portal lymph node metastases originate from the liver metastasis and not, as previously thought, from primary colon cancer.

Original languageEnglish (US)
Pages (from-to)2556-2561
Number of pages6
JournalClinical Cancer Research
Volume6
Issue number6
StatePublished - Jun 1 2000

Fingerprint

Liver Neoplasms
Colonic Neoplasms
Neoplasm Metastasis
Liver
Nude Mice
Lymph Nodes
Abdomen
Colon
Neoplasms
Drainage

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Rashidi, B., Gamagami, R., Sasson, A. R., Sun, F. X., Geller, J., Moossa, A. R., & Hoffman, R. M. (2000). An orthotopic mouse model of remetastasis of human colon cancer liver metastasis. Clinical Cancer Research, 6(6), 2556-2561.

An orthotopic mouse model of remetastasis of human colon cancer liver metastasis. / Rashidi, Babak; Gamagami, Reza; Sasson, Aaron R; Sun, Fang Xian; Geller, Jack; Moossa, A. R.; Hoffman, Robert M.

In: Clinical Cancer Research, Vol. 6, No. 6, 01.06.2000, p. 2556-2561.

Research output: Contribution to journalArticle

Rashidi, B, Gamagami, R, Sasson, AR, Sun, FX, Geller, J, Moossa, AR & Hoffman, RM 2000, 'An orthotopic mouse model of remetastasis of human colon cancer liver metastasis', Clinical Cancer Research, vol. 6, no. 6, pp. 2556-2561.
Rashidi B, Gamagami R, Sasson AR, Sun FX, Geller J, Moossa AR et al. An orthotopic mouse model of remetastasis of human colon cancer liver metastasis. Clinical Cancer Research. 2000 Jun 1;6(6):2556-2561.
Rashidi, Babak ; Gamagami, Reza ; Sasson, Aaron R ; Sun, Fang Xian ; Geller, Jack ; Moossa, A. R. ; Hoffman, Robert M. / An orthotopic mouse model of remetastasis of human colon cancer liver metastasis. In: Clinical Cancer Research. 2000 ; Vol. 6, No. 6. pp. 2556-2561.
@article{730b4de585604ebc973aea57f90dfbf1,
title = "An orthotopic mouse model of remetastasis of human colon cancer liver metastasis",
abstract = "Whether liver metastases from colon cancer are capable of metastasizing to other sites is an important question in surgical oncology. To answer this question, we have developed a highly metastatic orthotopic transplant model of a liver metastasis from a human colon cancer patient in nude mice that targets the liver and lymph nodes. The metastatic human tumor was transplanted in athymic nude mice by surgical orthotopic implantation (SOI) of a liver metastasis from a colon cancer patient. The human colon tumor was then subsequently implanted in the colon by SOI or, in an additional series of nude mice, in the liver by surgical hepatic implantation (SHI). The mice were then explored over time for lymph node involvement beginning 10 days after implantation. After SOI, 100{\%} of the animals had liver metastasis within 10 days, and subsequently, 19 days after SOI, all lymph nodes draining the liver were involved with metastasis without any retroperitoneal or lung tissue involvement. After SHI, all sites of lymphatic drainage of the liver, including portal, celiac, and mediastinal lymph nodes, were massively involved by metastasis in 100{\%} of the animals as early as 10 days after tumor implantation on the liver. The results of this study demonstrate that liver metastases from colon cancer are capable of remetastasizing to other sites. This study thus suggests that in colon cancer patients with liver metastasis, mediastinal, celiac, and portal lymph node metastases originate from the liver metastasis and not, as previously thought, from primary colon cancer.",
author = "Babak Rashidi and Reza Gamagami and Sasson, {Aaron R} and Sun, {Fang Xian} and Jack Geller and Moossa, {A. R.} and Hoffman, {Robert M.}",
year = "2000",
month = "6",
day = "1",
language = "English (US)",
volume = "6",
pages = "2556--2561",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "6",

}

TY - JOUR

T1 - An orthotopic mouse model of remetastasis of human colon cancer liver metastasis

AU - Rashidi, Babak

AU - Gamagami, Reza

AU - Sasson, Aaron R

AU - Sun, Fang Xian

AU - Geller, Jack

AU - Moossa, A. R.

AU - Hoffman, Robert M.

PY - 2000/6/1

Y1 - 2000/6/1

N2 - Whether liver metastases from colon cancer are capable of metastasizing to other sites is an important question in surgical oncology. To answer this question, we have developed a highly metastatic orthotopic transplant model of a liver metastasis from a human colon cancer patient in nude mice that targets the liver and lymph nodes. The metastatic human tumor was transplanted in athymic nude mice by surgical orthotopic implantation (SOI) of a liver metastasis from a colon cancer patient. The human colon tumor was then subsequently implanted in the colon by SOI or, in an additional series of nude mice, in the liver by surgical hepatic implantation (SHI). The mice were then explored over time for lymph node involvement beginning 10 days after implantation. After SOI, 100% of the animals had liver metastasis within 10 days, and subsequently, 19 days after SOI, all lymph nodes draining the liver were involved with metastasis without any retroperitoneal or lung tissue involvement. After SHI, all sites of lymphatic drainage of the liver, including portal, celiac, and mediastinal lymph nodes, were massively involved by metastasis in 100% of the animals as early as 10 days after tumor implantation on the liver. The results of this study demonstrate that liver metastases from colon cancer are capable of remetastasizing to other sites. This study thus suggests that in colon cancer patients with liver metastasis, mediastinal, celiac, and portal lymph node metastases originate from the liver metastasis and not, as previously thought, from primary colon cancer.

AB - Whether liver metastases from colon cancer are capable of metastasizing to other sites is an important question in surgical oncology. To answer this question, we have developed a highly metastatic orthotopic transplant model of a liver metastasis from a human colon cancer patient in nude mice that targets the liver and lymph nodes. The metastatic human tumor was transplanted in athymic nude mice by surgical orthotopic implantation (SOI) of a liver metastasis from a colon cancer patient. The human colon tumor was then subsequently implanted in the colon by SOI or, in an additional series of nude mice, in the liver by surgical hepatic implantation (SHI). The mice were then explored over time for lymph node involvement beginning 10 days after implantation. After SOI, 100% of the animals had liver metastasis within 10 days, and subsequently, 19 days after SOI, all lymph nodes draining the liver were involved with metastasis without any retroperitoneal or lung tissue involvement. After SHI, all sites of lymphatic drainage of the liver, including portal, celiac, and mediastinal lymph nodes, were massively involved by metastasis in 100% of the animals as early as 10 days after tumor implantation on the liver. The results of this study demonstrate that liver metastases from colon cancer are capable of remetastasizing to other sites. This study thus suggests that in colon cancer patients with liver metastasis, mediastinal, celiac, and portal lymph node metastases originate from the liver metastasis and not, as previously thought, from primary colon cancer.

UR - http://www.scopus.com/inward/record.url?scp=0034042625&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034042625&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 2556

EP - 2561

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 6

ER -