An introduction to single molecule biophysics

Research output: Book/ReportBook

2 Citations (Scopus)

Abstract

This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

Original languageEnglish (US)
PublisherCRC Press
Number of pages234
ISBN (Electronic)9781351333122
ISBN (Print)9781439806944
DOIs
StatePublished - Jan 1 2017

Fingerprint

Nanopores
Biophysics
biophysics
Optical Tweezers
Molecules
Molecular Dynamics Simulation
Genomics
DNA Replication
DNA Sequence Analysis
molecules
Fluorescence
Technology
Delivery of Health Care
DNA
deoxyribonucleic acid
Optical tweezers
sequencing
Transcription
imaging techniques
Microscopes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)
  • Engineering(all)

Cite this

An introduction to single molecule biophysics. / Lyubchenko, Yuri L.

CRC Press, 2017. 234 p.

Research output: Book/ReportBook

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