An insulator element and condensed chromatin region separate the chicken β-globin locus from an independently regulated erythroid-specific folate receptor gene

Marie Noëlle Prioleau, Pascale Nony, Melanie A Simpson, Gary Felsenfeld

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

We have identified a folate receptor gene upstream of the chicken β-globin locus and separated from it by a 16 kbp region of silent chromatin. We find that this receptor is expressed only at a stage of erythroid differentiation (CFU-E) preceding the activation of β-globin genes, consistent with the role of folate receptors in proliferation. This discovery raises the question of how these two loci are regulated during erythropoiesis. Our data suggest that the folate receptor gene and the β-globin locus are regulated independently. We show that a 3.3 kbp DNA region upstream of the folate receptor gene is sufficient to induce strong expression of a transgene in CFU-E stage cells. We also find that the region between the β-globin locus and the folate receptor gene is fully methylated and condensed at this stage of differentiation. Its 3' boundary coincides with the 5' β-globin insulator. We speculate that the 5' β-globin boundary element might be important for the proper regulation of two adjacent domains activated at two different stages during differentiation.

Original languageEnglish (US)
Pages (from-to)4035-4048
Number of pages14
JournalEMBO Journal
Volume18
Issue number14
DOIs
StatePublished - Jul 15 1999

Fingerprint

Insulator Elements
Globins
Folic Acid
Chromatin
Chickens
Genes
Erythroid Precursor Cells
Erythropoiesis
Transgenes
Transcriptional Activation
Chemical activation
DNA

Keywords

  • Boundary
  • Condensed chromatin
  • Domain
  • Methylation
  • β-globin locus

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

An insulator element and condensed chromatin region separate the chicken β-globin locus from an independently regulated erythroid-specific folate receptor gene. / Prioleau, Marie Noëlle; Nony, Pascale; Simpson, Melanie A; Felsenfeld, Gary.

In: EMBO Journal, Vol. 18, No. 14, 15.07.1999, p. 4035-4048.

Research output: Contribution to journalArticle

Prioleau, Marie Noëlle ; Nony, Pascale ; Simpson, Melanie A ; Felsenfeld, Gary. / An insulator element and condensed chromatin region separate the chicken β-globin locus from an independently regulated erythroid-specific folate receptor gene. In: EMBO Journal. 1999 ; Vol. 18, No. 14. pp. 4035-4048.
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