An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis

Wenming Wang, Xiaohua Yang, Samantha Tangchaiburana, Roland Ndeh, Jonathan E. Markham, Yoseph Tsegaye, Teresa M. Dunn, Guo Liang Wang, Maria Bellizzi, James F. Parsons, Danielle Morrissey, Janis E. Bravo, Daniel V. Lynch, Shunyuan Xiao

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

The Arabidopsis thaliana resistance gene RPW8 triggers the hypersensitive response (HR) to restrict powdery mildew infection via the salicylic acid-dependent signaling pathway. To further understand how RPW8 signaling is regulated, we have conducted a genetic screen to identify mutations enhancing RPW8-mediated HR-like cell death (designated erh). Here, we report the isolation and characterization of the Arabidopsis erh 1 mutant, in which the At2g37940 locus is knocked out by a T-DNA insertion. Loss of function of ERH1 results in salicylic acid accumulation, enhanced transcription of RPW8 and RPW8-dependent spontaneous HR-like cell death in leaf tissues, and reduction in plant stature. Sequence analysis suggests that ERH1 may encode the long-sought Arabidopsis functional homolog of yeast and protozoan inositolphosphorylceramide synthase (IPCS), which converts ceramide to inositolphosphorylceramide. Indeed, ERH1 is able to rescue the yeast aur1 mutant, which lacks the IPCS, and the erh1 mutant plants display reduced (∼53% of wild type) levels of leaf IPCS activity, indicating that ERH1 encodes a plant IPCS. Consistent with its biochemical function, the erh1 mutation causes ceramide accumulation in plants expressing RPW8. These data reinforce the concept that sphingolipid metabolism (specifically, ceramide accumulation) plays an important role in modulating plant programmed cell death associated with defense.

Original languageEnglish (US)
Pages (from-to)3163-3179
Number of pages17
JournalPlant Cell
Volume20
Issue number11
DOIs
StatePublished - Nov 1 2008

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Plant Cells
Arabidopsis
Cell Death
apoptosis
ceramides
Ceramides
hypersensitive response
Salicylic Acid
salicylic acid
mutants
cell death
Yeasts
yeasts
mutation
sphingolipids
Mutation
Sphingolipids
powdery mildew
Sequence Analysis
Protozoa

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis. / Wang, Wenming; Yang, Xiaohua; Tangchaiburana, Samantha; Ndeh, Roland; Markham, Jonathan E.; Tsegaye, Yoseph; Dunn, Teresa M.; Wang, Guo Liang; Bellizzi, Maria; Parsons, James F.; Morrissey, Danielle; Bravo, Janis E.; Lynch, Daniel V.; Xiao, Shunyuan.

In: Plant Cell, Vol. 20, No. 11, 01.11.2008, p. 3163-3179.

Research output: Contribution to journalArticle

Wang, W, Yang, X, Tangchaiburana, S, Ndeh, R, Markham, JE, Tsegaye, Y, Dunn, TM, Wang, GL, Bellizzi, M, Parsons, JF, Morrissey, D, Bravo, JE, Lynch, DV & Xiao, S 2008, 'An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis', Plant Cell, vol. 20, no. 11, pp. 3163-3179. https://doi.org/10.1105/tpc.108.060053
Wang, Wenming ; Yang, Xiaohua ; Tangchaiburana, Samantha ; Ndeh, Roland ; Markham, Jonathan E. ; Tsegaye, Yoseph ; Dunn, Teresa M. ; Wang, Guo Liang ; Bellizzi, Maria ; Parsons, James F. ; Morrissey, Danielle ; Bravo, Janis E. ; Lynch, Daniel V. ; Xiao, Shunyuan. / An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis. In: Plant Cell. 2008 ; Vol. 20, No. 11. pp. 3163-3179.
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