An inhibitory monoclonal antibody against human tissue factor

Steven D Carson, S. E. Ross, R. Bach, A. Guha

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

We obtained a hybridoma using immune spleen cells from a mouse injected with human brain tissue factor that had been purified on a factor VII-agarose affinity column. This monoclonal IgG1, HTF1-7B8, inhibits tissue factor procoagulant activity. The concentration of HTF1-7B8 producing half-maximal inhibition is influenced by the concentration of factor VIIa, suggesting that the antibody and enzyme compete for the cofactor. The antibody was successfully used to detect both human and bovine tissue factor on nitrocellulose dot blots, indicating that the epitope recognized by this antibody is conserved in both species. This antibody clearly reveals tissue factor on a Western blot. An HTF1-7B8 affinity column was used to purify tissue factor from both human brain and placenta. The electrophoretic mobilities in polyacrylamide gels in the presence of sodium dodecyl sulfate (SDS) and the amino acid compositions of the purified tissue factor from brain and placenta are indistinguishable, as are their specific procoagulant activities in reconstituted systems. This antibody will be useful for immunopurification and characterization of tissue factor structure and mechanism.

Original languageEnglish (US)
Pages (from-to)490-493
Number of pages4
JournalBlood
Volume70
Issue number2
StatePublished - Jan 1 1987

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Thromboplastin
Monoclonal Antibodies
Antibodies
Brain
Placenta
Factor VIIa
Electrophoretic mobility
Factor VII
Collodion
Coenzymes
Hybridomas
Sodium Dodecyl Sulfate
Sepharose
Epitopes
Spleen
Immunoglobulin G
Western Blotting
Amino Acids
Chemical analysis

ASJC Scopus subject areas

  • Immunology
  • Biochemistry
  • Hematology
  • Cell Biology

Cite this

Carson, S. D., Ross, S. E., Bach, R., & Guha, A. (1987). An inhibitory monoclonal antibody against human tissue factor. Blood, 70(2), 490-493.

An inhibitory monoclonal antibody against human tissue factor. / Carson, Steven D; Ross, S. E.; Bach, R.; Guha, A.

In: Blood, Vol. 70, No. 2, 01.01.1987, p. 490-493.

Research output: Contribution to journalArticle

Carson, SD, Ross, SE, Bach, R & Guha, A 1987, 'An inhibitory monoclonal antibody against human tissue factor', Blood, vol. 70, no. 2, pp. 490-493.
Carson SD, Ross SE, Bach R, Guha A. An inhibitory monoclonal antibody against human tissue factor. Blood. 1987 Jan 1;70(2):490-493.
Carson, Steven D ; Ross, S. E. ; Bach, R. ; Guha, A. / An inhibitory monoclonal antibody against human tissue factor. In: Blood. 1987 ; Vol. 70, No. 2. pp. 490-493.
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