An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer

Pi-Wan Cheng, Thomas F. Boat

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.

Original languageEnglish (US)
Pages (from-to)276-282
Number of pages7
JournalAnalytical Biochemistry
Volume85
Issue number1
DOIs
StatePublished - Jan 1 1978

Fingerprint

Galactosamine
Glucosamine
Buffers
Ninhydrin
Hexosamines
Amino Acids
1-Propanol
Borates
Citric Acid
Acids
2-amino-2-deoxygalactitol
2-amino-2-deoxyglucitol

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer. / Cheng, Pi-Wan; Boat, Thomas F.

In: Analytical Biochemistry, Vol. 85, No. 1, 01.01.1978, p. 276-282.

Research output: Contribution to journalArticle

@article{b654cf4fb7244101baa4cdd290bf97f1,
title = "An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer",
abstract = "Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15{\%} ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.",
author = "Pi-Wan Cheng and Boat, {Thomas F.}",
year = "1978",
month = "1",
day = "1",
doi = "10.1016/0003-2697(78)90300-7",
language = "English (US)",
volume = "85",
pages = "276--282",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer

AU - Cheng, Pi-Wan

AU - Boat, Thomas F.

PY - 1978/1/1

Y1 - 1978/1/1

N2 - Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.

AB - Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.

UR - http://www.scopus.com/inward/record.url?scp=0017840721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017840721&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(78)90300-7

DO - 10.1016/0003-2697(78)90300-7

M3 - Article

VL - 85

SP - 276

EP - 282

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -