An expanded genetic code in mammalian cells with a functional quadruplet codon

Wei Niu, Peter G. Schultz, Jiantao Guo

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We have utilized in vitro evolution to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to a quadruplet codon in both bacterial and mammalian cells. This approach will facilitate the creation of an optimized and standardized system for the genetic incorporation of unnatural amino acids using quadruplet codons, which will allow the biosynthesis of biopolymers that contain multiple unnatural building blocks.

Original languageEnglish (US)
Pages (from-to)1640-1645
Number of pages6
JournalACS Chemical Biology
Volume8
Issue number7
DOIs
StatePublished - Jul 19 2013

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Genetic Code
Codon
Cells
Amino Acids
Biopolymers
Biosynthesis
Transfer RNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

Cite this

An expanded genetic code in mammalian cells with a functional quadruplet codon. / Niu, Wei; Schultz, Peter G.; Guo, Jiantao.

In: ACS Chemical Biology, Vol. 8, No. 7, 19.07.2013, p. 1640-1645.

Research output: Contribution to journalArticle

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