An Exome-seq Based Tool for Mapping and Selection of Candidate Genes in Maize Deletion Mutants

Shangang Jia, Kyla Morton, Chi Zhang, David Holding

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite the large number of genomic and transcriptomic resources in maize, there is still much to learn about the function of genes in developmental and biochemical processes. Some maize mutants that were generated by gamma-irradiation showed clear segregation for the kernel phenotypes in B73 × Mo17 F2 ears. To better understand the functional genomics of kernel development, we developed a mapping and gene identification pipeline, bulked segregant exome sequencing (BSEx-seq), to map mutants with kernel phenotypes including opaque endosperm and reduced kernel size. BSEx-seq generates and compares the sequence of the exon fraction from mutant and normal plant F2 DNA pools. The comparison can derive mapping peaks, identify deletions within the mapping peak, and suggest candidate genes within the deleted regions. We then used the public kernel-specific expression data to narrow down the list of candidate genes/mutations and identified deletions ranging from several kb to more than 1 Mb. A full deletion allele of the Opaque-2 gene was identified in mutant 531, which occurs within a ∼200-kb deletion. Opaque mutant 1486 has a 6248-bp deletion in the mapping interval containing two candidate genes encoding RNA-directed DNA methylation 4 (RdDM4) and AMP-binding protein, respectively. This study demonstrates the efficiency and cost-effectiveness of BSEx-seq for causal mutation mapping and candidate gene selection, providing a new option in mapping-by-sequencing for maize functional genomics studies.

Original languageEnglish (US)
Pages (from-to)439-450
Number of pages12
JournalGenomics, Proteomics and Bioinformatics
Volume16
Issue number6
DOIs
StatePublished - Dec 1 2018

Fingerprint

Exome
Maize
Mutant
Deletion
Zea mays
Genes
Gene
Chromosome Mapping
Sequencing
Genomics
kernel
Biochemical Phenomena
Functional Genomics
Plant DNA
Developmental Genes
Phenotype
Endosperm
Sequence Deletion
DNA Methylation
Adenosine Monophosphate

Keywords

  • Bulked segregation analysis
  • Exome-seq
  • Maize functional genomics
  • Mapping-by-sequencing
  • Opaque mutant

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Computational Mathematics

Cite this

An Exome-seq Based Tool for Mapping and Selection of Candidate Genes in Maize Deletion Mutants. / Jia, Shangang; Morton, Kyla; Zhang, Chi; Holding, David.

In: Genomics, Proteomics and Bioinformatics, Vol. 16, No. 6, 01.12.2018, p. 439-450.

Research output: Contribution to journalArticle

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