An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein

Satyanarayana Tatineni, Anthony J. McMechan, Everlyne N. Wosula, Stephen N. Wegulo, Robert A. Graybosch, Roy French, Gary L. Hein

Research output: Contribution to journalArticle

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Abstract

Eriophyid mite-transmitted, multipartite, negative-sense RNA plant viruses with membrane-bound spherical virions are classified in the genus Emaravirus. We report here that the eriophyid mite-transmitted Wheat mosaic virus (WMoV), an Emaravirus, contains eight genomic RNA segments, the most in a known negative-sense RNA plant virus. Remarkably, two RNA 3 consensus sequences, encoding the nucleocapsid protein, were found with 12.5% sequence divergence, while no heterogeneity was observed in the consensus sequences of additional genomic RNA segments. The RNA-dependent RNA polymerase, glycoprotein precursor, nucleocapsid, and P4 proteins of WMoV exhibited limited sequence homology with the orthologous proteins of other emaraviruses, while proteins encoded by additional genomic RNA segments displayed no significant homology with proteins reported in GenBank, suggesting that the genus Emaravirus evolved further with a divergent octapartite genome. Phylogenetic analyses revealed that WMoV formed an evolutionary link between members of the Emaravirus genus and the family Bunyaviridae. Furthermore, genomic-length virus- and virus-complementary (vc)-sense strands of all WMoV genomic RNAs accumulated asymmetrically in infected wheat, with 10- to 20-fold more virus-sense genomic RNAs than vc-sense RNAs. These data further confirm the octapartite negative-sense polarity of the WMoV genome. In WMoV-infected wheat, subgenomic-length mRNAs of vc sense were detected for genomic RNAs 3, 4, 7, and 8 but not for other RNA species, suggesting that the open reading frames present in the complementary sense of genomic RNAs are expressed through subgenomic- or near-genomic-length vc-sense mRNAs.

Original languageEnglish (US)
Pages (from-to)11834-11845
Number of pages12
JournalJournal of virology
Volume88
Issue number20
DOIs
StatePublished - Jan 1 2014

Fingerprint

nucleocapsid proteins
Nucleocapsid Proteins
Plant Viruses
Eriophyidae
Mites
plant viruses
Mosaic Viruses
Emaravirus
High Plains virus
Triticum
RNA
genomics
Viruses
RNA Viruses
viruses
Consensus Sequence
consensus sequence
Bunyaviridae
Genome
RNA Replicase

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Tatineni, S., McMechan, A. J., Wosula, E. N., Wegulo, S. N., Graybosch, R. A., French, R., & Hein, G. L. (2014). An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein. Journal of virology, 88(20), 11834-11845. https://doi.org/10.1128/JVI.01901-14

An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein. / Tatineni, Satyanarayana; McMechan, Anthony J.; Wosula, Everlyne N.; Wegulo, Stephen N.; Graybosch, Robert A.; French, Roy; Hein, Gary L.

In: Journal of virology, Vol. 88, No. 20, 01.01.2014, p. 11834-11845.

Research output: Contribution to journalArticle

Tatineni, S, McMechan, AJ, Wosula, EN, Wegulo, SN, Graybosch, RA, French, R & Hein, GL 2014, 'An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein', Journal of virology, vol. 88, no. 20, pp. 11834-11845. https://doi.org/10.1128/JVI.01901-14
Tatineni, Satyanarayana ; McMechan, Anthony J. ; Wosula, Everlyne N. ; Wegulo, Stephen N. ; Graybosch, Robert A. ; French, Roy ; Hein, Gary L. / An eriophyid mite-transmitted plant virus contains eight genomic RNA segments with unusual heterogeneity in the nucleocapsid protein. In: Journal of virology. 2014 ; Vol. 88, No. 20. pp. 11834-11845.
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