An enzyme-linked immunoassay for the cartilage proteoglycan

Stephen Israel Rennard, K. Kimata, B. Dusemund, H. J. Barrach, J. Wilczek, J. H. Kimura, V. C. Hascall

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

Original languageEnglish (US)
Pages (from-to)399-406
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume207
Issue number2
DOIs
StatePublished - Apr 1 1981

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Cartilage
Proteoglycans
Immunoenzyme Techniques
Enzymes
Rats
Molecular Weight
Molecular weight
Chondrosarcoma
Immune Sera
Assays
Serum

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Rennard, S. I., Kimata, K., Dusemund, B., Barrach, H. J., Wilczek, J., Kimura, J. H., & Hascall, V. C. (1981). An enzyme-linked immunoassay for the cartilage proteoglycan. Archives of Biochemistry and Biophysics, 207(2), 399-406. https://doi.org/10.1016/0003-9861(81)90047-3

An enzyme-linked immunoassay for the cartilage proteoglycan. / Rennard, Stephen Israel; Kimata, K.; Dusemund, B.; Barrach, H. J.; Wilczek, J.; Kimura, J. H.; Hascall, V. C.

In: Archives of Biochemistry and Biophysics, Vol. 207, No. 2, 01.04.1981, p. 399-406.

Research output: Contribution to journalArticle

Rennard, SI, Kimata, K, Dusemund, B, Barrach, HJ, Wilczek, J, Kimura, JH & Hascall, VC 1981, 'An enzyme-linked immunoassay for the cartilage proteoglycan', Archives of Biochemistry and Biophysics, vol. 207, no. 2, pp. 399-406. https://doi.org/10.1016/0003-9861(81)90047-3
Rennard SI, Kimata K, Dusemund B, Barrach HJ, Wilczek J, Kimura JH et al. An enzyme-linked immunoassay for the cartilage proteoglycan. Archives of Biochemistry and Biophysics. 1981 Apr 1;207(2):399-406. https://doi.org/10.1016/0003-9861(81)90047-3
Rennard, Stephen Israel ; Kimata, K. ; Dusemund, B. ; Barrach, H. J. ; Wilczek, J. ; Kimura, J. H. ; Hascall, V. C. / An enzyme-linked immunoassay for the cartilage proteoglycan. In: Archives of Biochemistry and Biophysics. 1981 ; Vol. 207, No. 2. pp. 399-406.
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