An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication

M. J. Heller, L. J. Burgart, C. J. TenEyck, M. E. Anderson, Timothy Charles Greiner, R. A. Robinson

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A method was developed for fast and efficient isolation of DNA from formalin-fixed, paraffin-embedded tissue sections for subsequent use in PCRs and DNA hybridization assays. The method relies on the use of a sonicating water bath to disrupt tissue samples to which a small amount of micro-sized glass beads have been added. The sonicating glass beads provide fast and efficient physical shearing of fixed tissue sections, allowing for quick release and solubilization of the DNA. The extraction process from paraffin section to amplifiable target DNA takes 30 minutes. The methodd eliminates the need for repetitive solvent extractions and exhaustive proteinase K digestion. PCR amplification of human genomic and viral target sequencess was successfully carried out on DNA isolated from a number of different types of normal and infected tissues.

Original languageEnglish (US)
Pages (from-to)372-377
Number of pages6
JournalBioTechniques
Volume11
Issue number3
StatePublished - Oct 21 1991

Fingerprint

Sonication
Paraffin
Formaldehyde
Tissue
DNA
Glass
Endopeptidase K
Polymerase Chain Reaction
Solvent extraction
Baths
Shearing
Amplification
Digestion
Assays
Water

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Heller, M. J., Burgart, L. J., TenEyck, C. J., Anderson, M. E., Greiner, T. C., & Robinson, R. A. (1991). An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication. BioTechniques, 11(3), 372-377.

An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication. / Heller, M. J.; Burgart, L. J.; TenEyck, C. J.; Anderson, M. E.; Greiner, Timothy Charles; Robinson, R. A.

In: BioTechniques, Vol. 11, No. 3, 21.10.1991, p. 372-377.

Research output: Contribution to journalArticle

Heller, MJ, Burgart, LJ, TenEyck, CJ, Anderson, ME, Greiner, TC & Robinson, RA 1991, 'An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication', BioTechniques, vol. 11, no. 3, pp. 372-377.
Heller MJ, Burgart LJ, TenEyck CJ, Anderson ME, Greiner TC, Robinson RA. An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication. BioTechniques. 1991 Oct 21;11(3):372-377.
Heller, M. J. ; Burgart, L. J. ; TenEyck, C. J. ; Anderson, M. E. ; Greiner, Timothy Charles ; Robinson, R. A. / An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication. In: BioTechniques. 1991 ; Vol. 11, No. 3. pp. 372-377.
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