An avidin-biotin enhanced dot-immunobinding assay for the detection of Mycoplasma gallisepticum and M. synoviae serum antibodies in chickens.

D. R. Cummins, D. L. Reynolds, K. R. Rhoades

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A dot-immunobinding assay was enhanced by the incorporation of avidin and biotin reagents into the test system (DAB assay). This assay was used to detect serum antibodies to Mycoplasma gallisepticum (MG) and M. synoviae (MS) from chickens. Serum samples were tested by rapid serum plate (RSP), hemagglutination-inhibition (HI), and DAB assay methods. These results were compared. The DAB assay was at least 20 times more sensitive in detecting antibodies for MS and at least 75 times more sensitive in detecting antibodies for MG than the HI test. The DAB assay was as specific as the HI test. The DAB assay was also more sensitive and specific than the RSP test. Some cross-reactions occurred when low dilutions of high-titer sera were used in the DAB assay. Parameters for determining negative, suspicious, and positive samples were established. The DAB assay for MG and MS may have several applications, including use as a screening test and a confirmatory test.

Original languageEnglish (US)
Pages (from-to)36-43
Number of pages8
JournalAvian diseases
Volume34
Issue number1
DOIs
StatePublished - Jan 1 1990

Fingerprint

Mycoplasma gallisepticum
Mycoplasma synoviae
avidin
Avidin
biotin
Biotin
Chickens
chickens
antibodies
Antibodies
Hemagglutination Inhibition Tests
assays
Serum
hemagglutination inhibition test
Cross Reactions
Hemagglutination
testing
hemagglutination
cross reaction
screening

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Immunology and Microbiology(all)

Cite this

An avidin-biotin enhanced dot-immunobinding assay for the detection of Mycoplasma gallisepticum and M. synoviae serum antibodies in chickens. / Cummins, D. R.; Reynolds, D. L.; Rhoades, K. R.

In: Avian diseases, Vol. 34, No. 1, 01.01.1990, p. 36-43.

Research output: Contribution to journalArticle

@article{beedbd8af2c84ba5a02e4017af48ce16,
title = "An avidin-biotin enhanced dot-immunobinding assay for the detection of Mycoplasma gallisepticum and M. synoviae serum antibodies in chickens.",
abstract = "A dot-immunobinding assay was enhanced by the incorporation of avidin and biotin reagents into the test system (DAB assay). This assay was used to detect serum antibodies to Mycoplasma gallisepticum (MG) and M. synoviae (MS) from chickens. Serum samples were tested by rapid serum plate (RSP), hemagglutination-inhibition (HI), and DAB assay methods. These results were compared. The DAB assay was at least 20 times more sensitive in detecting antibodies for MS and at least 75 times more sensitive in detecting antibodies for MG than the HI test. The DAB assay was as specific as the HI test. The DAB assay was also more sensitive and specific than the RSP test. Some cross-reactions occurred when low dilutions of high-titer sera were used in the DAB assay. Parameters for determining negative, suspicious, and positive samples were established. The DAB assay for MG and MS may have several applications, including use as a screening test and a confirmatory test.",
author = "Cummins, {D. R.} and Reynolds, {D. L.} and Rhoades, {K. R.}",
year = "1990",
month = "1",
day = "1",
doi = "10.2307/1591331",
language = "English (US)",
volume = "34",
pages = "36--43",
journal = "Avian Diseases",
issn = "0005-2086",
publisher = "American Association of Avian Pathologists",
number = "1",

}

TY - JOUR

T1 - An avidin-biotin enhanced dot-immunobinding assay for the detection of Mycoplasma gallisepticum and M. synoviae serum antibodies in chickens.

AU - Cummins, D. R.

AU - Reynolds, D. L.

AU - Rhoades, K. R.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - A dot-immunobinding assay was enhanced by the incorporation of avidin and biotin reagents into the test system (DAB assay). This assay was used to detect serum antibodies to Mycoplasma gallisepticum (MG) and M. synoviae (MS) from chickens. Serum samples were tested by rapid serum plate (RSP), hemagglutination-inhibition (HI), and DAB assay methods. These results were compared. The DAB assay was at least 20 times more sensitive in detecting antibodies for MS and at least 75 times more sensitive in detecting antibodies for MG than the HI test. The DAB assay was as specific as the HI test. The DAB assay was also more sensitive and specific than the RSP test. Some cross-reactions occurred when low dilutions of high-titer sera were used in the DAB assay. Parameters for determining negative, suspicious, and positive samples were established. The DAB assay for MG and MS may have several applications, including use as a screening test and a confirmatory test.

AB - A dot-immunobinding assay was enhanced by the incorporation of avidin and biotin reagents into the test system (DAB assay). This assay was used to detect serum antibodies to Mycoplasma gallisepticum (MG) and M. synoviae (MS) from chickens. Serum samples were tested by rapid serum plate (RSP), hemagglutination-inhibition (HI), and DAB assay methods. These results were compared. The DAB assay was at least 20 times more sensitive in detecting antibodies for MS and at least 75 times more sensitive in detecting antibodies for MG than the HI test. The DAB assay was as specific as the HI test. The DAB assay was also more sensitive and specific than the RSP test. Some cross-reactions occurred when low dilutions of high-titer sera were used in the DAB assay. Parameters for determining negative, suspicious, and positive samples were established. The DAB assay for MG and MS may have several applications, including use as a screening test and a confirmatory test.

UR - http://www.scopus.com/inward/record.url?scp=0025069556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025069556&partnerID=8YFLogxK

U2 - 10.2307/1591331

DO - 10.2307/1591331

M3 - Article

C2 - 2322229

AN - SCOPUS:0025069556

VL - 34

SP - 36

EP - 43

JO - Avian Diseases

JF - Avian Diseases

SN - 0005-2086

IS - 1

ER -