An assessment of doubled haploid production in soft red winter wheat by wheat x corn wide crosses

H. Sharma, Yiqi Yang, H. Ohm

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Feasibility of wheat x corn hybridization to produce doubled haploids (DHs) for soft red winter wheat (SRWW) breeding was assessed. Seven intervarietal wheat hybrids were pollinated with the same corn genotype (Seneca 60) in a greenhouse, followed by 2,4-D application, 6-24 hours post-pollination, embryo rescue at the end of third week after pollination and chromosome doubling by colchicine in the media at 1-2 leaf stage of the plantlets. The plantlets were transferred to soil, maintained in a growth chamber for two weeks and then grown in the greenhouse. There was no seed set without 2,4-D treatment and 81-90% (mean 87%), including proliferated ovaries, with 100 ppm 2,4-D treatment. Wheat hybrids differed significantly for percent seed set with corn pollination and for percent seeds with embryos but not for differentiation of cultured embryos. Haploid seed development was better in coin envelops than in glassine bags. 16% seeds had embryos, 37% embryos had normal organogenesis, 50% seedlings survived to maturity and 25% of them set seed. Wheat hybrid 5156 was the best with 86% seed set, 35% seeds with embryos, 54% embryos producing plantlets of which 57% survived to maturity and 50% of the survivors set seed. Overall, ir 100 DHs are needed for selection of desirable segregants, enough intervarietal wheat hybrid seed would be required to have 289 plants of an intervarietal cross for pollination with corn.

Original languageEnglish (US)
Pages (from-to)269-275
Number of pages7
JournalCereal Research Communications
Volume30
Issue number3-4
StatePublished - Dec 1 2002

Fingerprint

soft red winter wheat
Haploidy
doubled haploids
embryo (plant)
Triticum
Zea mays
Seeds
seed set
wheat
corn
Embryonic Structures
Pollination
2,4-D
plantlets
2,4-Dichlorophenoxyacetic Acid
pollination
seeds
greenhouses
cross pollination
colchicine

Keywords

  • Corn
  • Crossing bags
  • Haploidy
  • Maize
  • Soft red winter wheat
  • Wheat
  • Wide crossing

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics

Cite this

An assessment of doubled haploid production in soft red winter wheat by wheat x corn wide crosses. / Sharma, H.; Yang, Yiqi; Ohm, H.

In: Cereal Research Communications, Vol. 30, No. 3-4, 01.12.2002, p. 269-275.

Research output: Contribution to journalArticle

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abstract = "Feasibility of wheat x corn hybridization to produce doubled haploids (DHs) for soft red winter wheat (SRWW) breeding was assessed. Seven intervarietal wheat hybrids were pollinated with the same corn genotype (Seneca 60) in a greenhouse, followed by 2,4-D application, 6-24 hours post-pollination, embryo rescue at the end of third week after pollination and chromosome doubling by colchicine in the media at 1-2 leaf stage of the plantlets. The plantlets were transferred to soil, maintained in a growth chamber for two weeks and then grown in the greenhouse. There was no seed set without 2,4-D treatment and 81-90{\%} (mean 87{\%}), including proliferated ovaries, with 100 ppm 2,4-D treatment. Wheat hybrids differed significantly for percent seed set with corn pollination and for percent seeds with embryos but not for differentiation of cultured embryos. Haploid seed development was better in coin envelops than in glassine bags. 16{\%} seeds had embryos, 37{\%} embryos had normal organogenesis, 50{\%} seedlings survived to maturity and 25{\%} of them set seed. Wheat hybrid 5156 was the best with 86{\%} seed set, 35{\%} seeds with embryos, 54{\%} embryos producing plantlets of which 57{\%} survived to maturity and 50{\%} of the survivors set seed. Overall, ir 100 DHs are needed for selection of desirable segregants, enough intervarietal wheat hybrid seed would be required to have 289 plants of an intervarietal cross for pollination with corn.",
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