An approach to sequence DNA without tagging

Sanjun Niu, Ravi F. Saraf

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microarray technology is playing an increasingly important role in biology and medicine and its application to genomics for gene expression analysis has already reached the market with a variety of commercially available instruments. In these combinatorial analysis methods, known probe single-strand DNA (ssDNA) 'primers' are attached in clusters of typically 100 μm × 100 μm pixels. Each pixel of the array has a slightly different sequence. On exposure to 'unknown' target ssDNA, the pixels with the right complementary probe ssDNA sequence convert to double-stranded DNA (dsDNA) by a hybridization reaction. To transduct the conversion of the pixel to dsDNA, the target ssDNA is labelled with a photoluminescent tag during the polymerase chain reaction (PCR) amplification process. Due to the statistical distribution of the tags in the target ssDNA, it becomes significantly difficult to implement these methods as a diagnostic tool in a pathology laboratory. A method to sequence DNA without tagging the molecule is developed. The fabrication process is compatible with current microelectronics and (emerging) soft-material fabrication technologies, allowing the method to be integrable with micro-electromechanical systems (MEMS) and lab-on-a-chip devices. An estimated sensitivity of 10-12 g on a 1 cm2 device area is obtained.

Original languageEnglish (US)
Pages (from-to)778-782
Number of pages5
JournalSmart Materials and Structures
Volume11
Issue number5
DOIs
StatePublished - Oct 1 2002

Fingerprint

strands
marking
DNA
deoxyribonucleic acid
Pixels
pixels
combinatorial analysis
Fabrication
Lab-on-a-chip
lab-on-a-chip devices
Polymerase chain reaction
DNA sequences
Pathology
Microarrays
polymerase chain reaction
primers
fabrication
Gene expression
Microelectronics
gene expression

ASJC Scopus subject areas

  • Signal Processing
  • Civil and Structural Engineering
  • Atomic and Molecular Physics, and Optics
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Electrical and Electronic Engineering

Cite this

An approach to sequence DNA without tagging. / Niu, Sanjun; Saraf, Ravi F.

In: Smart Materials and Structures, Vol. 11, No. 5, 01.10.2002, p. 778-782.

Research output: Contribution to journalArticle

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