An approach to revealing blood fluke life cycles, taxonomy, and diversity

Provision of key reference data including DNA sequence from single life cycle stages

Sara V. Brant, Jess A T Morgan, Gerald M. Mkoji, Scott D. Snyder, R. P V Jayanthe Rajapakse, Eric S. Loker

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Revealing diversity among extant blood flukes, and the patterns of relationships among them, has been hindered by the difficulty of determining if specimens described from different life cycle stages, hosts, geographic localities, and times represent the same or different species. Persistent collection of all available life cycle stages and provision of exact collection localities, host identification, reference DNA sequences for the parasite, and voucher specimens eventually will provide the framework needed to piece together individual life cycles and facilitate reconciliation with classical taxonomic descriptions, including those based on single life cycle stages. It also provides a means to document unique or rare species that might only ever be recovered from a single life cycle stage. With an emphasis on the value of new information from field collections of any available life cycle stages, here we provide data for several blood fluke cercariae from freshwater snails from Kenya, Uganda, and Australia. Similar data are provided for adult worms of Macrobilharzia macrobilharzia and miracidia of Bivitellobilharzia nairi. Some schistosome and sanguinicolid cercariae that we recovered have peculiar morphological features, and our phylogenetic analyses (18S and 28S rDNA and mtDNA CO1) suggest that 2 of the new schistosome specimens likely represent previously unknown lineages. Our results also provide new insights into 2 of the 4 remaining schistosome genera yet to be extensively characterized with respect to their position in molecular phylogenies, Macrobilharua and Bivitellobilharzia. The accessibility of each life cycle stage is likely to vary dramatically from one parasite species to the next, and our examples validate the potential usefulness of information gleaned from even one such stage, whatever it might be.

Original languageEnglish (US)
Pages (from-to)77-88
Number of pages12
JournalJournal of Parasitology
Volume92
Issue number1
DOIs
StatePublished - Feb 1 2006

Fingerprint

blood flukes
Trematoda
Life Cycle Stages
life cycle (organisms)
life cycle
blood
developmental stages
taxonomy
nucleotide sequences
DNA
Schistosoma
cercariae
Cercaria
parasite
Parasites
miracidia
parasites
phylogeny
Uganda
type collections

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

An approach to revealing blood fluke life cycles, taxonomy, and diversity : Provision of key reference data including DNA sequence from single life cycle stages. / Brant, Sara V.; Morgan, Jess A T; Mkoji, Gerald M.; Snyder, Scott D.; Rajapakse, R. P V Jayanthe; Loker, Eric S.

In: Journal of Parasitology, Vol. 92, No. 1, 01.02.2006, p. 77-88.

Research output: Contribution to journalArticle

Brant, Sara V. ; Morgan, Jess A T ; Mkoji, Gerald M. ; Snyder, Scott D. ; Rajapakse, R. P V Jayanthe ; Loker, Eric S. / An approach to revealing blood fluke life cycles, taxonomy, and diversity : Provision of key reference data including DNA sequence from single life cycle stages. In: Journal of Parasitology. 2006 ; Vol. 92, No. 1. pp. 77-88.
@article{810ddd9876054d0da1bebd2a7543ee79,
title = "An approach to revealing blood fluke life cycles, taxonomy, and diversity: Provision of key reference data including DNA sequence from single life cycle stages",
abstract = "Revealing diversity among extant blood flukes, and the patterns of relationships among them, has been hindered by the difficulty of determining if specimens described from different life cycle stages, hosts, geographic localities, and times represent the same or different species. Persistent collection of all available life cycle stages and provision of exact collection localities, host identification, reference DNA sequences for the parasite, and voucher specimens eventually will provide the framework needed to piece together individual life cycles and facilitate reconciliation with classical taxonomic descriptions, including those based on single life cycle stages. It also provides a means to document unique or rare species that might only ever be recovered from a single life cycle stage. With an emphasis on the value of new information from field collections of any available life cycle stages, here we provide data for several blood fluke cercariae from freshwater snails from Kenya, Uganda, and Australia. Similar data are provided for adult worms of Macrobilharzia macrobilharzia and miracidia of Bivitellobilharzia nairi. Some schistosome and sanguinicolid cercariae that we recovered have peculiar morphological features, and our phylogenetic analyses (18S and 28S rDNA and mtDNA CO1) suggest that 2 of the new schistosome specimens likely represent previously unknown lineages. Our results also provide new insights into 2 of the 4 remaining schistosome genera yet to be extensively characterized with respect to their position in molecular phylogenies, Macrobilharua and Bivitellobilharzia. The accessibility of each life cycle stage is likely to vary dramatically from one parasite species to the next, and our examples validate the potential usefulness of information gleaned from even one such stage, whatever it might be.",
author = "Brant, {Sara V.} and Morgan, {Jess A T} and Mkoji, {Gerald M.} and Snyder, {Scott D.} and Rajapakse, {R. P V Jayanthe} and Loker, {Eric S.}",
year = "2006",
month = "2",
day = "1",
doi = "10.1645/GE-3515.1",
language = "English (US)",
volume = "92",
pages = "77--88",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "1",

}

TY - JOUR

T1 - An approach to revealing blood fluke life cycles, taxonomy, and diversity

T2 - Provision of key reference data including DNA sequence from single life cycle stages

AU - Brant, Sara V.

AU - Morgan, Jess A T

AU - Mkoji, Gerald M.

AU - Snyder, Scott D.

AU - Rajapakse, R. P V Jayanthe

AU - Loker, Eric S.

PY - 2006/2/1

Y1 - 2006/2/1

N2 - Revealing diversity among extant blood flukes, and the patterns of relationships among them, has been hindered by the difficulty of determining if specimens described from different life cycle stages, hosts, geographic localities, and times represent the same or different species. Persistent collection of all available life cycle stages and provision of exact collection localities, host identification, reference DNA sequences for the parasite, and voucher specimens eventually will provide the framework needed to piece together individual life cycles and facilitate reconciliation with classical taxonomic descriptions, including those based on single life cycle stages. It also provides a means to document unique or rare species that might only ever be recovered from a single life cycle stage. With an emphasis on the value of new information from field collections of any available life cycle stages, here we provide data for several blood fluke cercariae from freshwater snails from Kenya, Uganda, and Australia. Similar data are provided for adult worms of Macrobilharzia macrobilharzia and miracidia of Bivitellobilharzia nairi. Some schistosome and sanguinicolid cercariae that we recovered have peculiar morphological features, and our phylogenetic analyses (18S and 28S rDNA and mtDNA CO1) suggest that 2 of the new schistosome specimens likely represent previously unknown lineages. Our results also provide new insights into 2 of the 4 remaining schistosome genera yet to be extensively characterized with respect to their position in molecular phylogenies, Macrobilharua and Bivitellobilharzia. The accessibility of each life cycle stage is likely to vary dramatically from one parasite species to the next, and our examples validate the potential usefulness of information gleaned from even one such stage, whatever it might be.

AB - Revealing diversity among extant blood flukes, and the patterns of relationships among them, has been hindered by the difficulty of determining if specimens described from different life cycle stages, hosts, geographic localities, and times represent the same or different species. Persistent collection of all available life cycle stages and provision of exact collection localities, host identification, reference DNA sequences for the parasite, and voucher specimens eventually will provide the framework needed to piece together individual life cycles and facilitate reconciliation with classical taxonomic descriptions, including those based on single life cycle stages. It also provides a means to document unique or rare species that might only ever be recovered from a single life cycle stage. With an emphasis on the value of new information from field collections of any available life cycle stages, here we provide data for several blood fluke cercariae from freshwater snails from Kenya, Uganda, and Australia. Similar data are provided for adult worms of Macrobilharzia macrobilharzia and miracidia of Bivitellobilharzia nairi. Some schistosome and sanguinicolid cercariae that we recovered have peculiar morphological features, and our phylogenetic analyses (18S and 28S rDNA and mtDNA CO1) suggest that 2 of the new schistosome specimens likely represent previously unknown lineages. Our results also provide new insights into 2 of the 4 remaining schistosome genera yet to be extensively characterized with respect to their position in molecular phylogenies, Macrobilharua and Bivitellobilharzia. The accessibility of each life cycle stage is likely to vary dramatically from one parasite species to the next, and our examples validate the potential usefulness of information gleaned from even one such stage, whatever it might be.

UR - http://www.scopus.com/inward/record.url?scp=33645540500&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645540500&partnerID=8YFLogxK

U2 - 10.1645/GE-3515.1

DO - 10.1645/GE-3515.1

M3 - Article

VL - 92

SP - 77

EP - 88

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 1

ER -